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SAE0116

Heparanase-1 pre-activated human

recombinant, expressed in HEK 293 cells

Synonym(s):

Endo-glucoronidase, HPA, HPA1, HPR1, HPSE, HPSE1, HSE1, Heparanase

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Size/SKUAvailabilityPrice
10 μg

Available to ship TODAYfromMILWAUKEE

$665.00

About This Item

EC Number:
NACRES:
NA.26
UNSPSC Code:
12352202
Assay:
≥95.0% (SDS-PAGE)
Recombinant:
expressed in HEK 293 cells

$665.00


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recombinant

expressed in HEK 293 cells

assay

≥95.0% (SDS-PAGE)

form

lyophilized powder

impurities

≤1.00 EU/μg (LAL)

UniProt accession no.

shipped in

ambient

storage temp.

−20°C

General description

Recombinant human heparanase-1 is expressed in human HEK 293 cells as an active heterodimer glycoprotein containing a 50 kDa subunit and a 8 kDa subunit. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein. The protein is produced with no artificial tags.

Biochem/physiol Actions

Heparanase-1 (heparanase) is the only heparan sulfate degrading endoglycosidase. It is involved in the regulation of multiple biological processes that increase tumor growth, metastasis, angiogenesis and inflammation. Heparanase is involved in ECM remodeling and heparan sulfate-linked biological molecules release, including cytokines and growth factors. Heparanase cleaves the heparan sulfate side chains of heparan sulfate proteoglycans into fragments of 10–20 sugar units.
Heparanase is initially translated as a preproenzyme containing a signal peptide. Cleavage of the signal sequence yields a latent 65 kDa pro-heparanase, which must undergo further processing for activity. Mature active heparanase is a heterodimer consisting an N-terminal 8 kDa subunit and a C-terminal 50 kDa subunit.

Other Notes

One heparanase activity unit is the amount of heparanase required to cleave 1 pmol of pentasaccharide to disaccharide and trisaccharide in one minute, at 37 °C, pH 5.0.

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ECM002SAE0075SAE0078
description

recombinant, expressed in HEK 293 cells

description

recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

description

recombinant, ≥10 U/mg, expressed in HEK 293 cells

description

recombinant, ≥1,300 pmol/min/μg, expressed in HEK 293 cells

assay

≥95.0% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

liquid

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

UniProt accession no.

Q9Y251

UniProt accession no.

P07996

UniProt accession no.

P12821

UniProt accession no.

P14780

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

ambient

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice


Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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