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SAE0196

Achromopeptidase from bacteria

free of DNA contaminants, suitable for Microbiome research

Synonym(s):

Bacterial Protease

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Size/SKUAvailabilityPrice
50 mg

Available to ship TODAYfromMILWAUKEE

$213.00

About This Item

NACRES:
NA.54
UNSPSC Code:
12352204
Specific activity:
≥1000 U/mg
Biological source:
bacterial

$213.00


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biological source

bacterial

Quality Segment

form

lyophilized powder

specific activity

≥1000 U/mg

feature

DNA free

technique(s)

DNA extraction: suitable

input

bacteria and yeast sample(s)

suitability

suitable for cell lysis

application(s)

cell analysis

shipped in

ambient

storage temp.

−20°C

General description

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme, as well as Gram-negative organisms. Achromopeptidase has been shown to contain two bacteriolytic proteases. This product is free of detectable microbial DNA and is suitable for microbiome research

Application

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme, as well as Gram-negative organisms. Achromopeptidase has been shown to contain two bacteriolytic proteases.

The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminants are essential. This product undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.

Biochem/physiol Actions

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. Optimum pH is 8.5-9.

Features and Benefits

Contains two bacteriolytic proteases for efficient lysis of Gram-positive bacteria and Gram-negative organisms

Free of detectable microbial DNA, ensuring the accuracy of metagenomics and other culture-independent sequencing methods

Undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA

Optimum pH of 8.5-9 allows for use in a wide range of microbiome studies

Other Notes

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

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This Item
A3547A3422C1990
description

free of DNA contaminants, suitable for Microbiome research

description

lyophilized powder, ≥1,000 units/mg solid

description

partially purified powder, ≥20,000 units/mg solid

description

-

biological source

bacterial

biological source

bacterial (LYSOBACTER SP. BACTERIA M497-1)

biological source

-

biological source

-

specific activity

≥1000 U/mg

specific activity

≥1,000 units/mg solid

specific activity

≥20,000 units/mg solid

specific activity

-

technique(s)

DNA extraction: suitable

technique(s)

cell based assay: suitable

technique(s)

-

technique(s)

-

application(s)

cell analysis

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

form

lyophilized powder

form

lyophilized powder

form

partially purified powder

form

powder

suitability

suitable for cell lysis

suitability

suitable for cell lysis

suitability

-

suitability

-


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Pictograms

Health hazard

Signal Word

Danger

Hazard Codes

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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