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SRP0256

RB Active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Synonym(s):

OSRC, RB1, Retinoblastoma 1, Retinoblastoma-associated protein, p105-Rb

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200 μg
Usually ships in 2 weeks.
$727.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
Form:
aqueous solution
Assay:
≥80% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli
Mol wt:
43.5 kDa

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biological source

human

recombinant

expressed in E. coli

assay

≥80% (SDS-PAGE)

form

aqueous solution

mol wt

43.5 kDa

packaging

pkg of 200 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... RB1(5925)

General description

The gene encoding Retinoblastoma-associated protein (RB1) is a tumor suppressor gene localized on human chromosome 13q14.2. Human recombinant RB protein (773-end) with N-terminal GST tag (GenBank Accession No. NM_000321), expressed in a E. coli expression system.

Application

Retinoblastoma-associated protein (RB1) has been used in cell activity assay. It is also used as a kinase substrate for the kinase assay and in Western blotting.

Biochem/physiol Actions

Retinoblastoma-associated protein (RB1) modulates the cell cycle. It acts as a repressor of transcription mediated by E2 factor (E2F) and influences transcriptional silencing. RB1 modulates mitochondrial activities and has a role in the organisation of heterochromatin. Mutations in the gene encoding this protein have been associated with childhood retinoblastoma.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

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RB Active human recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Sigma-Aldrich

SRP0256

RB Active human

Retinoblastoma protein human recombinant, expressed in insect cells, ≥70% (SDS-PAGE)

Sigma-Aldrich

SRP2081

Retinoblastoma protein human

Anti-RB antibody produced in rabbit

Sigma-Aldrich

SAB5700023

Anti-RB antibody produced in rabbit

Anti- RB antibody produced in rabbit

Sigma-Aldrich

SAB5701471

Anti- RB antibody produced in rabbit

description

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

description

recombinant, expressed in insect cells, ≥70% (SDS-PAGE)

description

-

description

-

recombinant

expressed in E. coli

recombinant

expressed in insect cells

recombinant

-

recombinant

-

assay

≥80% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

-

assay

-

biological source

human

biological source

human

biological source

rabbit

biological source

rabbit

concentration

>0.02 mg/mL

concentration

400 μg/mL

concentration

0.72 mg/ml

concentration

1.5 mg/mL

mol wt

43.5 kDa

mol wt

~107.5 kDa

mol wt

106

mol wt

-

form

aqueous solution

form

frozen liquid

form

buffered aqueous solution

form

liquid

Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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