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SRP0273

PDE8A1 Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

Synonym(s):

PDE8, PDE8A, Phosphodiesterase 8A1

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10 μg
Usually ships in 2 weeks.
$811.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
Specific activity:
≥437 pmol/min-μg
Assay:
≥70% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in baculovirus infected insect cells
Concentration:
>0.02 mg/mL

$811.00

Usually ships in 2 weeks.
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biological source

human

recombinant

expressed in baculovirus infected insect cells

assay

≥70% (SDS-PAGE)

form

aqueous solution

specific activity

≥437 pmol/min-μg

mol wt

119 kDa

packaging

pkg of 10 μg

concentration

>0.02 mg/mL

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PDE8A(5151)

General description

PDE8A (phosphodiesterase 8A) is one of the two subfamilies of PDE8 protein family, the other being PDE8B. PDE8 shows wide range of tissue expression, and is especially abundant in testis. PDE8A contains a Per, ARNT and Sim (PAS) signaling domain in its N-terminal. PDE8s contain receiver (REC) domain, N-glycosylation, N-myristoylation, amidation consensus sites, and potential kinase substrate sites for PKC (protein kinase), PKA and casein kinase.
Human PDE8A1 (GenBank Accession No. NM_002605), full length with N-terminal GST tag, MW = 119kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

PDE8 (phosphodiesterase 8) are cAMP-specific enzymes, and they play a role in various processes such as in T-cell activation regulation, chemotaxis of activated lymphocytes, control of testosterone synthesis in Leydig cells, initiation of glucose-induced biphasic insulin response, steroidogenesis, and excitation–contraction coupling. rs2304418 variant in this gene is linked with lower mRNA expression of PDE8A, which in turn is linked with reduced HIV-1 (human immunodeficiency virus) replication. The expression of this protein is significantly high in macrophages, and is controlled by miR-145-5p. PDE8A promotes HIV-1 replication in macrophages and this is controlled at the level of reverse transcription.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Other Notes

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C

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PDE8A1 Active human recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

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SRP0273

PDE8A1 Active human

PDE1B Active human recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

Sigma-Aldrich

SRP0258

PDE1B Active human

PDE9A2 Active human recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

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SRP0274

PDE9A2 Active human

PDE4A1A Active human recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

Sigma-Aldrich

SRP0262

PDE4A1A Active human

description

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

description

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

description

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

description

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

biological source

human

biological source

human

biological source

human

biological source

human

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

≥80% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

specific activity

≥437 pmol/min-μg

specific activity

≥2000 units/μg

specific activity

≥97 pmol/min-μg

specific activity

-

Gene Information

human ... PDE8A(5151)

Gene Information

human ... PDE1B(5153)

Gene Information

human ... PDE9A(5152)

Gene Information

human ... PDE4A(5141)

form

aqueous solution

form

aqueous solution

form

aqueous solution

form

aqueous solution

concentration

>0.02 mg/mL

concentration

>0.02 mg/mL

concentration

>0.02 mg/mL

concentration

>0.02 mg/mL

Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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