The supplied amount of protein is 10 μg. The concentration of the protein is approximately 0.1 μg/μL, but may vary slightly from lot to lot. This minimum purity is 70% as determined by densitometry. The PP2Aa molecular weight is approximately 38 kDa and the PP2R1A molecular weight is approximately 65 kDa as shown in the image below:
SRP0736
PP2A ALPHA/PPP2R1A Complex, Active
recombinant
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| Size/SKU | Availability | Price |
|---|---|---|
10 μg | Usually ships in 2 weeks. | $562.00 |
About This Item
NACRES:
NA.77
UNSPSC Code:
12352202
Form:
frozen liquid
Assay:
70-95% (SDS-PAGE)
Recombinant:
expressed in baculovirus infected Sf9 cells
Mol wt:
~38 kDa, ~65 kDa
$562.00
Usually ships in 2 weeks.
recombinant
expressed in baculovirus infected Sf9 cells
assay
70-95% (SDS-PAGE)
form
frozen liquid
specific activity
3400-4600 nmol/min·mg (BIOMOL Green)
mol wt
~38 kDa, ~65 kDa
concentration
0.1 μg/μL
accession no.
NM_002715, NM_014225
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PP2A(5524), PPP2R1A(5518)
Application
Useful for Phosphatase assay and Western blot.
Biochem/physiol Actions
Recombinant full length human PP2Aalpha and human PPP2R1A were expressed by Baculovirus in Sf9 insect cells using an N-terminal His tag. PP2Aalpha is also known as Protein phosphatase 2A active subunit. PP2A is one of the four major Ser/Thr phosphatases and is a human tumor suppressor that inhibits cellular transformation. PP2A alpha gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase-2A (1). Protein phosphatase 2A holoenzyme is a heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. PTP2A alpha can alter the substrate specificity of PP2A leading to enhanced phosphotyrosine phosphatase activity and decreased phosphoserine phosphatase activity (2).
Preparation Note
Formulated in 50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, with 25% glycerol.
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
1 of 1
This Item | |||
|---|---|---|---|
| description recombinant | description clone 4E6, purified immunoglobulin, buffered aqueous solution | description targeting human PPP2R1A | description targeting mouse Ppp2r1a |
| assay 70-95% (SDS-PAGE) | assay - | assay - | assay - |
| recombinant expressed in baculovirus infected Sf9 cells | recombinant - | recombinant - | recombinant - |
| concentration 0.1 μg/μL | concentration - | concentration - | concentration - |
| mol wt ~65 kDa, ~38 kDa | mol wt - | mol wt - | mol wt - |
| form frozen liquid | form buffered aqueous solution | form lyophilized powder | form lyophilized powder |
| Gene Information human ... PP2A(5524), PPP2R1A(5518) | Gene Information human ... PPP2R1A(5518) | Gene Information human ... PPP2R1A(5518) | Gene Information mouse ... PPP2R1A(51792), Ppp2r1a(51792) |
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2 - vPvM
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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