Further dilution in PBS with 10% FBS is a suitable option. Storing the solution with a stabilizer is recommended. As FBS contains BSA, this would be a suitable stabilizer. Avoid repeated freeze/thaw cycles. Store in working aliquots at -20°C to -80°C.
SRP3093
IL-4 human
Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym(s):
B cell stimulating factor (BSF-1), BCDF, BCGF
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| Size/SKU | Availability | Price |
|---|---|---|
20 μg | Usually ships in 2 weeks. | $453.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
$453.00
Usually ships in 2 weeks.
biological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC), ≥98% (SDS-PAGE)
form
lyophilized
potency
≤0.2 ng/mL ED50
mol wt
14.9 kDa
packaging
pkg of 20 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
off-white to yellow
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... IL4(3565)
General description
IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant human IL-4 is a 14.9 kDa globular protein containing 129 amino acid residues.
Application
Biochem/physiol Actions
IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Recombinant human IL-4 is a 14.9 kDa globular protein containing 129 amino acid residues.
Physical form
Lyophilized with no additives.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.
Other Notes
MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS
1 of 1
This Item | |||
|---|---|---|---|
| description Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture | description recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC) | description Animal-component free, recombinant, expressed in E. coli, suitable for cell culture | description Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC) |
| assay ≥98% (SDS-PAGE), ≥98% (HPLC) | assay ≥95% (SDS-PAGE), ≥95% (HPLC) | assay ≥95% (HPLC) | assay ≥98% (SDS-PAGE), ≥98% (HPLC) |
| biological source human | biological source human | biological source human | biological source human |
| recombinant expressed in E. coli | recombinant expressed in HEK 293 cells | recombinant expressed in E. coli | recombinant expressed in E. coli |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable | technique(s) - |
| form lyophilized | form lyophilized | form (Lyophilized with no additives from a 0.2µ filtered solution) | form lyophilized |
| mol wt 14.9 kDa | mol wt 52.5 kDa | mol wt 15.5 kDa | mol wt 17.4 kDa |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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After the initial reconstitution in water, can this be diluted in PBS with 10% FBS?
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