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SRP3142

PF-4 (CXCL4) human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

CXCL4, Ironplact, Oncostatin A, Platelet Factor-4

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20 μg
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$337.00
1 mg
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$3,910.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352202

$337.00

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biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

form

lyophilized

potency

1.0-10.0 ng/mL

mol wt

7.8 kDa

packaging

pkg of 20 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

P02776

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PF4(5196)

General description

Platelet factor-4 (PF-4) is a CXC chemokine that is expressed in megakaryocytes and stored in the alpha-granules of platelets. The gene encoding this protein is localized on human chromosome 4. Recombinant human PF-4 is a 7.8kDa protein containing 70 amino acid residues, including the four highly conserved residues present in CXC chemokines.

Biochem/physiol Actions

Platelet factor-4 (PF-4) is chemotactic towards neutrophils and monocytes and has been shown to inhibit angiogenesis. This protein associates with heparin and neutralizes it. It also neutralizes various negatively charged proteoglycans.

Physical form

Lyophilized with no additives.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Other Notes

EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ LIATLKNGRK ICLDLQAPLY KKIIKKLLES

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PF-4 (CXCL4) human recombinant, expressed in E. coli, &#8805;98% (SDS-PAGE), &#8805;98% (HPLC), suitable for cell culture

Sigma-Aldrich

SRP3142

PF-4 (CXCL4) human

MIP-1 beta (CCL4) human recombinant, expressed in E. coli, &#8805;98% (SDS-PAGE), &#8805;98% (HPLC), suitable for cell culture

Sigma-Aldrich

SRP3115

MIP-1 beta (CCL4) human

ENA-78 (8-78) human recombinant, expressed in E. coli, &#8805;98% (SDS-PAGE), &#8805;98% (HPLC), suitable for cell culture

Sigma-Aldrich

SRP3030

ENA-78 (8-78) human

IL-8 (77aa) (CXCL8) human recombinant, expressed in E. coli, &#8805;98% (SDS-PAGE), &#8805;98% (HPLC), suitable for cell culture

Sigma-Aldrich

SRP3098

IL-8 (77aa) (CXCL8) human

description

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

description

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

description

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

description

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

biological source

human

biological source

human

biological source

human

biological source

human

form

lyophilized

form

lyophilized

form

lyophilized

form

lyophilized

Gene Information

human ... PF4(5196)

Gene Information

human ... CCL4(6351)

Gene Information

human ... CXCL5(6374)

Gene Information

human ... IL8(3576)

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Questions

  1. can this protein work on mouse?

    1 answer

    1. This product is tested for activity using human fibroblasts. A brief search of the literature could not confirm that the human source protein would be fully bioactive in mice. However, product SRP3231 is PF-4 from mouse and is tested on human neutrophils. While it is likely that mouse cells would respond to the human chemokine, it would be up to the end user to confirm suitability. See the links below to review a sample Certificate of Analysis for each product.

      SRP3142 Human PL-4
      https://www.sigmaaldrich.com/coa/SIGMA/SRP3142/101543

      SRP3231 Mouse PF-4
      https://www.sigmaaldrich.com/coa/SIGMA/SRP3231/1208471

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