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SRP3177

TNF-α human

Animal-component free, recombinant, expressed in E. coli, suitable for cell culture

Synonym(s):

Cachectin, Cytotoxin, Differentiation-inducing factor (DIF), Necrosin, Tumor Necrosis Factor

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50 μg
Usually ships in 2 weeks.
$480.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352202

$480.00

Usually ships in 2 weeks.
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biological source

human

Quality Segment

recombinant

expressed in E. coli

assay

≥95% (SDS-PAGE)

form

lyophilized

potency

≤0.10 ng/mL ED50

mol wt

17.4 kDa

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤1.00 EU/μg endotoxin

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TNF(7124)

General description

TNF-α (tumor necrosis factor α) is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. The gene is mapped to human chromosome 6p21.3. Recombinant human TNF-α is a soluble 157 amino acid protein (17.4kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

Application

TNF-α (tumor necrosis factor α) human has been used as a positive control for induction of proinflammatory genes in human intestinal and hepatic cell lines.

Biochem/physiol Actions

TNF-α (tumor necrosis factor α) is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. However, it can be a tumor promoter as well as a tumor suppressor. TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, apoptosis and diabetes. It is also associated with mood and anxiety disorders.

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.4 + 0.5mM DTT.

Preparation Note

It is recommended to centrifuge the vial beforereconstitution. A stock solution may be prepared byreconstitution in sterile water to a concentration of0.1 mg/mL. The stock solution may be diluted furtherusing culture media.
Store the product at −20 °C. After reconstitution, theproduct can be stored at 2-8 °C for up to 1 month.For extended storage, freeze in working aliquots.Repeated freezing and thawing are not recommended.Do not store in a frost-free freezer.

Other Notes

VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL

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This Item
H8916GF023SAB5700627
TNF-α human Animal-component free, recombinant, expressed in E. coli, suitable for cell culture

Sigma-Aldrich

SRP3177

TNF-α human

Tumor Necrosis Factor-α human Xeno-free, recombinant, expressed in HEK 293 cells, suitable for cell culture

Sigma-Aldrich

H8916

Tumor Necrosis Factor-α human

Tumor Necrosis Factor-α Protein, Recombinant human Tumor Necrosis Factor-alpha (TNF-alpha) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells & certain other target cells.

Sigma-Aldrich

GF023

Tumor Necrosis Factor-α Protein, Recombinant human

Anti-TNF-α antibody produced in rabbit

Sigma-Aldrich

SAB5700627

Anti-TNF-α antibody produced in rabbit

description

Animal-component free, recombinant, expressed in E. coli, suitable for cell culture

description

Xeno-free, recombinant, expressed in HEK 293 cells, suitable for cell culture

description

Tumor Necrosis Factor-alpha (TNF-alpha) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells & certain other target cells.

description

-

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

>97% (SDS-PAGE)

assay

-

biological source

human

biological source

human

biological source

human

biological source

rabbit

recombinant

expressed in E. coli

recombinant

expressed in HEK 293 cells

recombinant

-

recombinant

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

immunohistochemistry: 1:50-1:200, immunofluorescence: 1:50-1:200, western blot: 1:1000-1:2000

Quality Level

300

Quality Level

200

Quality Level

100

Quality Level

100

form

lyophilized

form

lyophilized powder

form

-

form

buffered aqueous solution

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

Storage Class

11 - Combustible Solids

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