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VEGF human

Name: VEGF human
Brand: SIGMA
Price: 480 USD
Availability: InStock

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen, VPF, Vascular Endothelial Growth Factor-A

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10 μg	Usually ships in 2 weeks.	$480.00

About This Item

NACRES:

NA.32

UNSPSC Code:

12352202

$480.00

Usually ships in 2 weeks.

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Properties

biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

form

lyophilized

potency

1.0-8.0 ng/mL

mol wt

38.2 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

P15692

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... VEGFA(7422)

Description

General description

VEGF (vascular endothelial growth factor) signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Recombinant human VEGF is a 38.2kDa disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains.

Application

VEGF human has been used as a chemoattractant for HUVECs (human umbilical vein endothelial cells) migration assay.

Biochem/physiol Actions

VEGF (vascular endothelial growth factor) is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intraocular neovascular syndromes. Polymorphism in VEGF at C(-634)G is linked with diabetic retinopathy in type 2 diabetes.

Physical form

Lyophilized with no additives.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Other Notes

APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

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This Item	H9166	V7259	ABS82
Sigma-Aldrich SRP3182 VEGF human	Sigma-Aldrich H9166 Vascular Endothelial Growth Factor 165 human	Sigma-Aldrich V7259 Vascular Endothelial Growth Factor human	Sigma-Aldrich ABS82 Anti-VEGF Antibody
description Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture	description VEGF165, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture	description VEGF, recombinant, expressed in E. coli, powder, suitable for cell culture	description from rabbit, purified by affinity chromatography
assay ≥98% (SDS-PAGE), ≥98% (HPLC)	assay >95% (SDS-PAGE)	assay ≥98% (SDS-PAGE)	assay -
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) immunohistochemistry: suitable (paraffin), western blot: suitable
recombinant expressed in E. coli	recombinant expressed in HEK 293 cells	recombinant expressed in E. coli	recombinant -
biological source human	biological source human	biological source human	biological source rabbit
form lyophilized	form lyophilized powder	form powder	form -
impurities <0.1 EU/μg endotoxin, tested	impurities ≤1 EU/μg	impurities ≤0.1 EU/μg	impurities -

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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