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SRP3198

flt3-Ligand from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

Fms-related tyrosine kinase 3 ligand

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10 μg
Usually ships in 2 weeks.
$395.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
Form:
lyophilized
Assay:
≥98% (HPLC), ≥98% (SDS-PAGE)
Biological source:
mouse
Recombinant:
expressed in E. coli
Mol wt:
18.6 kDa

$395.00


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biological source

mouse

recombinant

expressed in E. coli

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

form

lyophilized

potency

5.0-8.0 ng/mL

mol wt

18.6 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... FLT3LG(14256)

General description

FMS-like tyrosine kinase 3 ligand (Flt3l), a cytokine, is also termed as Flk2/CD135. It is a hematopoietic growth factor. Recombinant murine Flt3-ligand is a soluble 18.6 kDa protein consisting of 163 amino acid residues.

Biochem/physiol Actions

Fms-like tyrosine kinase 3 (FLT3) ligand (FL), expressed by leukemic cells may help to increase the multiplication through an autocrine process.
Flt3l (FMS-like tyrosine kinase 3 ligand) acts as a stimulant of hematopoietic progenitor cell proliferation. It facilitates the commitment of lymphoid lineage, and aids in dendritic cell (DC) development. It is involved in myoblast differentiation, and drives skeletal muscle regeneration. It acts on p120RasGAP (rat sarcoma GTPase activating protein) and suppresses Erk (extracellular signal-regulated kinase) signaling, which in turn promotes myogenesis. In lymphoid tissues, it modulates DC homeostasis, which is facilitated by steady state as well as proliferative T-cells. Recombinant murine Flt3-Ligand is a soluble 18.6 kDa protein consisting of 163 amino acid residues.

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.8.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Other Notes

MTPDCYFSHS PISSNFKVKF RELTDHLLKD YPVTVAVNLQ DEKHCKALWS LFLAQRWIEQ LKTVAGSKMQ TLLEDVNTEI HFVTSCTFQP LPECLRFVQT NISHLLKDTC TQLLALKPCI GKACQNFSRC LEVQCQPDSS TLLPPRSPIA LEATELPEPR PRQ

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SRP3044SRP0349SRP3208
description

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

description

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

description

recombinant, expressed in baculovirus infected Sf9 cells, ≥80% (SDS-PAGE)

description

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

activity assay: suitable, inhibition assay: suitable

technique(s)

cell culture | mammalian: suitable

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in E. coli

biological source

mouse

biological source

human

biological source

human

biological source

mouse

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥80% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

mol wt

18.6 kDa

mol wt

17.6 kDa

mol wt

54.2 kDa

mol wt

15.1 kDa

form

lyophilized

form

lyophilized

form

aqueous solution

form

lyophilized


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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