As provided, this is the active form of mouse Granzyme B and not the proform. Activity level is determined by the ability to cleave a synthetic chromogenic Granzyme B substrate. For resuspension and solubility: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8 °C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (e.g., 0.1% BSA) and store in working aliquots at -20 °C to -80 °C.
SRP3202
Granzyme B from mouse
recombinant, expressed in Hi-5 Insect cells, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture
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| Size/SKU | Availability | Price |
|---|---|---|
10 μg | Usually ships in 2 weeks. | $409.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
$409.00
Usually ships in 2 weeks.
biological source
mouse
recombinant
expressed in Hi-5 Insect cells
assay
≥97% (HPLC), ≥97% (SDS-PAGE)
form
lyophilized
mol wt
28.9 kDa
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... GZMB(14939)
General description
Granzyme B is a cysteine protease found in the cytoplasmic granules of cytolytic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme B is required for the induction of target cell lysis, which occurs as part of cell mediated immune responses, and can activate apoptosis in target cells by both caspase dependent and caspase independent mechanisms. Proteolytic cleavage of substrates by Granzyme B takes place primarily after aspartic acid residues. Recombinant murine Granzyme B is a glycosylated 227 amino acid protein, comprising the mature active portion of the murine Granzyme B precursor. The apparent molecular weight is 28.9 kDa by mass spectrometry.
Biochem/physiol Actions
Granzyme B is a cysteine protease found in the cytoplasmic granules of cytolytic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme B is required for the induction of target cell lysis, which occurs as part of cell mediated immune responses, and can activate apoptosis in target cells by both caspase dependent and caspase independent mechanisms. Proteolytic cleavage of substrates by Granzyme B takes place primarily after aspartic acid residues. Recombinant murine Granzyme B is a glycosylated 227 amino acid protein, comprising the mature active portion of the murine Granzyme B precursor. The apparent molecular weight is 28.9 kDa by mass spectrometry.
Physical form
Lyophilized from 1x PBS.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Other Notes
IIGGHEVKPH SRPYMALLSI KDQQPEAICG GFLIREDFVL TAAHCEGSII NVTLGAHNIK EQEKTQQVIP MVKCIPHPDY NPKTFSNDIM LLKLKSKAKR TRAVRPLNLP RRNVNVKPGD VCYVAGWGRM APMGKYSNTL QEVELTVQKD RECESYFKNR YNKTNQICAG DPKTKRASFR GDSGGPLVCK KVAAGIVSYG YKDGSPPRAF TKVSSFLSWI KKTMKSS
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This Item | |||
|---|---|---|---|
| description recombinant, expressed in Hi-5 Insect cells, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture | description for serum, plasma and cell culture supernatant | description recombinant, expressed in insect cells, ≥90% (SDS-PAGE) | description The Granzyme B Inhibitor IV controls the biological activity of Granzyme B. This small molecule/inhibitor is primarily used for Cancer applications. |
| biological source mouse | biological source - | biological source human | biological source - |
| recombinant expressed in Hi-5 Insect cells | recombinant - | recombinant expressed in insect cells | recombinant - |
| technique(s) cell culture | mammalian: suitable | technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) - | technique(s) - |
| assay ≥97% (HPLC), ≥97% (SDS-PAGE) | assay - | assay ≥90% (SDS-PAGE) | assay ≥97% (HPLC) |
| form lyophilized | form - | form lyophilized powder | form solid |
| storage temp. −20°C | storage temp. −20°C | storage temp. −70°C | storage temp. −20°C |
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Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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Hello, Is this enzyme in its active form? What are the best condition for resuspension? Thank you.
1 answer-
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