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SRP3316

LIF human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

Synonym(s):

D factor, Differentiation-stimulating factor, Melanoma-derived LPL inhibitor (MLPLI)

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25 μg
Usually ships in 2 weeks.
$483.00

About This Item

NACRES:
NA.77
UNSPSC Code:
12352202

$483.00


Usually ships in 2 weeks.

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recombinant

expressed in E. coli

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

form

lyophilized

mol wt

19.6 kDa

impurities

endotoxin, tested

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... LIF(3976)

General description

Leukemia inhibitory factor (LIF) is a secreted pleiotropic glycoprotein which is a member of the gp130 signaling of interleukin-6 (IL-6) family cytokines. This protein was originally identified as a protein that prevented the growth of a leukemic cell line.
Recombinant human LIF is a 19.6 kDa protein containing 180 amino acids residues including three disulfide bonds.

Biochem/physiol Actions

Leukemia inhibitory factor (LIF) suppresses the differentiation of myoblasts, and inhibits the activation of caspase-3 and blocks the reduction in Bcl-xL (B-cell lymphoma-extra large) levels, thus, preventing doxorubicin-induced apoptosis. Along with transforming growth factor β2 (TGFβ2)/fibroblast growth factor 2 (FGF2), LIF regulates nephrogenesis. It facilitates tubular regeneration following acute kidney failure. It plays a crucial role in neuroprotective pathways induced by preconditioning-induced oxidative stress. LIF is capable of maintaining the totipotency of embryonic stem cells. It is also involved in controlling the differentiation of embryonic stem cells, hematopoietic and neuronal cells.

Physical form

Lyophilized with no additives

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

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LIF1050LIF1010LIF1005
description

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

description

50 µg, human recombinant LIF protein, expressed in E. coli, suitable for stem cell culture

description

10 µg, human recombinant LIF protein, expressed in E. coli, suitable for stem cell culture

description

5 µg, human recombinant LIF protein, expressed in E. coli, suitable for stem cell culture

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

assay

≥98% (SDS-PAGE), ≥98% (HPLC)

assay

>95% (SDS-PAGE)

assay

>95% (HPLC and SDS-PAGE)

assay

>95% (HPLC and SDS-PAGE)

form

lyophilized

form

liquid

form

liquid

form

liquid

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

UniProt accession no.

P15018

UniProt accession no.

P15018

UniProt accession no.

P15018

UniProt accession no.

P15018

mol wt

19.6 kDa

mol wt

-

mol wt

-

mol wt

-


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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