The structure of the material after purification is not assessed. While the native folding state cannot be guaranteed, the final purified flagellan can bind human and mouse TLR5 and activate TLR5 in cell culture.
SRP8029
Flagellin from Salmonella typhimurium
>95% (SDS-PAGE)
Synonym(s):
FliC, Intelectin-1
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| Size/SKU | Availability | Price |
|---|---|---|
10 μg | Usually ships in 2 weeks. | $285.00 |
About This Item
Form:
lyophilized
Assay:
>95% (SDS-PAGE)
Biological source:
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Mol wt:
~50 kDa by SDS-PAGE
biological source
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
assay
>95% (SDS-PAGE)
form
lyophilized
mol wt
~50 kDa by SDS-PAGE
packaging
pkg of 10 μg
impurities
<0.01 EU/μg endotoxin, tested
color
white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 ... fliC (STM1959)
General description
The encoded flagellin filament contains 55 kDa monomers.
Biochem/physiol Actions
It also stimulates the production of proinflammatory mediators : nitric oxide, interleukin 1 (IL-1), IL-6, IL-8, and tumor necrosis factor α.
Physical form
Lyophilized from PBS.
Preparation Note
Reconstitute with 100 μL deionized water.
1 of 1
This Item | |||
|---|---|---|---|
| description >95% (SDS-PAGE) | description IgG fraction of antiserum | description purified by gel-filtration chromatography | description Stimulates the activity of inducible nitric oxide synthase. |
| biological source Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 | biological source rabbit | biological source Salmonella enterica (Serotype typhimurium) | biological source - |
| assay >95% (SDS-PAGE) | assay - | assay - | assay - |
| form lyophilized | form liquid | form lyophilized powder | form lyophilized |
| mol wt ~50 kDa by SDS-PAGE | mol wt - | mol wt - | mol wt - |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Gene Information Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 ... fliC (STM1959)(1253480) | Gene Information - | Gene Information - | Gene Information - |
Storage Class
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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Hello! I wondered if this product is expressed from the host in its native folded state? I am interested in obtaining native folded flagellin... thanks for any insights you can provide in this matter!
1 answer-
Helpful?
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Good morning, I would like to have the sequence of this protein and the host where it was produced. Thank yo and best regards
1 answer-
This is native Flagellin purified from Salmonella typhimurium. The published sequence can be found here:
https://www.uniprot.org/uniprotkb/P06179/entryHelpful?
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