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Thrombin from human plasma

Name: Thrombin from human plasma
Brand: SIGMA
Price: 351 USD
Availability: InStock

lyophilized powder, ≥2800 NIH units/mg protein (E1%/280, 18.3)

Synonym(s):

Factor IIa

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Size/SKU	Availability	Price
250 units	Estimated to ship onSeptember 28, 2027fromMILWAUKEE	$351.00
1000 units	Estimated to ship onSeptember 28, 2027fromMILWAUKEE	$942.00

About This Item

CAS Number:

9002-04-4

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-648-7

MDL number:

MFCD00082072

EC Number:

3.4.21.5 (BRENDA, IUBMB)

Specific activity:

≥2800 NIH units/mg protein (E1%/280, 18.3)

$351.00

Estimated to ship onSeptember 28, 2027Details

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Properties

form

lyophilized powder

Quality Segment

200

specific activity

≥2800 NIH units/mg protein (E1%/280, 18.3)

mol wt

37.4 kDa

impurities

HIV, hepatitis B and hepatitis C, tested negative

UniProt accession no.

P00734

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Gene Information

human ... F2(2147)

Description

General description

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.

Application

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to evaluate the in vitro effect of low molecular weight heparin on thrombin generation in cirrhotic patients at different stages of liver disease.

Biochem/physiol Actions

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

Physical form

Lyophilized from 0.02 M Bis/Tris buffer, pH 6.5, 0.15 M NaCl and 0.1% PEG-8000

Analysis Note

The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Other Notes

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard

View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

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Show Differences

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This Item	T7572	T4393	T8885
Sigma-Aldrich T1063 Thrombin from human plasma	Sigma-Aldrich T7572 Thrombin from human plasma	Sigma-Aldrich T4393 Thrombin from human plasma	Sigma-Aldrich T8885 Thrombin from human plasma
description lyophilized powder, ≥2800 NIH units/mg protein (E1%/280, 18.3)	description 400-1000 NIH units/mg protein	description lyophilized powder, 1500-3500 NIH units/mg protein (E1%/280, 18.3), suitable for cell culture	description lyophilized powder, Suitable for routine use in the thrombin time test
Gene Information human ... F2(2147)	Gene Information human ... F2(2147)	Gene Information human ... F2(2147)	Gene Information human ... F2(2147)
specific activity ≥2800 NIH units/mg protein (E1%/280, 18.3)	specific activity 400-1000 NIH units/mg protein	specific activity 1500-3500 NIH units/mg protein (E1%/280, 18.3)	specific activity -
application(s) diagnostic assay manufacturing	application(s) -	application(s) -	application(s) -
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
mol wt 37.4 kDa	mol wt -	mol wt -	mol wt -

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Pictograms

GHS08,GHS07

Signal Word

Danger

Hazard Codes

H315,H319,H334,H335

Precautionary Statements

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class

11 - Combustible Solids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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Questions

8 months ago

Can thrombin T1063 be used for His tag removal? What are recommended buffer conditions for cleavage? What are recommended buffer conditions for storage?

1 answer
1. Technical Support
  
  6 months ago
  
  While the activity is defined by NIH units (clotting ability), this thrombin has been used by independent researchers to cleave fusion tags from recombinant proteins. For a potential protocol, please consult the literature. For example:
  https://pmc.ncbi.nlm.nih.gov/articles/PMC2172507/pdf/200405018.pdf
  https://www.cell.com/cell/fulltext/S0092-8674(21)00080-5?dgcid
  https://link.springer.com/content/pdf/10.1186/s10020-025-01339-7.pdf
  Storage recommendations can be found on the product datasheet:
  https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/167/952/t1063pis.pdf
  
  Helpful?

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