Key Documents

View All Documentation

Anti-TAP antibody, Mouse monoclonal

Name: Anti-TAP antibody, Mouse monoclonal
Brand: SIGMA
Price: 732 USD
Availability: InStock

clone 53H8, purified from hybridoma cell culture

Synonym(s):

Anti-TIP-associated protein

Select a Size

Change View

Size/SKU	Availability	Price
200 μL	Check Cart for Availability	$732.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD01865352

Conjugate:

unconjugated

Clone:

53H8, monoclonal

Application:

ARR, ELISA (i), ICC, IP, WB

Citations:

$732.00

Check Cart for Availability

Request a Custom Order

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

53H8, monoclonal

form

buffered aqueous solution

mol wt

antigen ~66 kDa

species reactivity

human

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 0.5-1 μg/mL using HeLa nuclear cell extract

isotype

IgG1

UniProt accession no.

Q9UBU9

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NXF1(10482)

Description

General description

Monoclonal Anti-TAP (mouse IgG1 isotype) is derived from the 53H8 hybridoma produced by the fusion of mouse myeloma cells (SP2/O) and splenocytes from Balb/c mice immunized with TAP.

TIP-associated protein (TAP), also known as nuclear export factor (NXF1), is encoded by the gene mapped to human chromosome 11q12.3. The encoded protein is a member of multigene family of evolutionarily conserved proteins. TAP contains non-canonical RNP-type RNA binding domain, four leucine-rich repeats and an NTF2-like domain. It also contains an ubiquitin-associated domain involved in the interaction with nucleoporins.

Immunogen

TAP (Tip Associated Protein).

Application

Anti-TAP antibody, mouse monoclonal may be used in:

enzyme linked immunosorbent assay (ELISA)
immunoblotting
immunoprecipitation
immunocytochemistry

Biochem/physiol Actions

Monoclonal Anti-TAP recognizes human TAP (~66 kDa).

TIP-associated protein (TAP) plays an important role in carrying mRNAs from the nucleus. Elevated expression of TAP or of SRp20, enhance the production of gammaretroviral Gag polyprotein. The encoded protein might facilitate the repression of two mutations caused by insertions of endogenous retroviruses into introns of cellular genes by regulating their mature transcript levels ∼2 fold.

Transporter associated with antigen processing (TAP) interacts with the RNA-binding protein Y14 protein, a participant of the mRNA-protein complex (mRNP) near the exon-exon junction of the spliced mRNAs. Formation of the TAP/p15 heterodimer is required for efficient nuclear pore complex binding and significantly enhances the ability of TAP to export mRNAs from the nucleus. In addition, TAP plays a central role in the nuclear export of (constitutive transport element) CTE containing viral mRNA. However, the recruitment of TAP to the two types of mRNA occurs by different mechanisms. TAP binds to the viral CTE directly, while the interaction with cellular mRNA is found to be indirectly.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	T0825	T1827	T1948
Sigma-Aldrich T1076 Anti-TAP antibody, Mouse monoclonal	Sigma-Aldrich T0825 Anti-Transportin 1 antibody, Mouse monoclonal	Sigma-Aldrich T1827 Anti -TBP antibody, Mouse monoclonal	Sigma-Aldrich T1948 Anti-TRF1 antibody, Mouse monoclonal
description clone 53H8, purified from hybridoma cell culture	description clone D45, purified from hybridoma cell culture	description clone 58C9, purified from hybridoma cell culture	description ~2 mg/mL, clone TRF-78, purified from hybridoma cell culture
species reactivity human	species reactivity mouse, canine, rat, human, bovine	species reactivity Sf9 cell line, human, yeast, Drosophila melanogaster	species reactivity human
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture
Gene Information human ... NXF1(10482)	Gene Information human ... TNPO1(3842) mouse ... Tnpo1(238799) rat ... Tnpo1(309126)	Gene Information Drosophila melanogaster ... Tbp(37476) human ... TBP(6908)	Gene Information human ... TERF1(7013)
biological source mouse	biological source mouse	biological source mouse	biological source mouse
clone 53H8, monoclonal	clone D45, monoclonal	clone 58C9	clone TRF-78, monoclonal

Still not finding the right product?

Try our Product Selector Tool to narrow your options

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c