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T1819

Trizma® hydrochloride solution

pH 7.0, BioPerformance Certified, 1 M, suitable for cell culture

1 of 1 reviewers took part in a promotion

Synonym(s):

Tris hydrochloride solution

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100 mL

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$52.90
1 L

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$99.20

About This Item

NACRES:
NA.25
UNSPSC Code:
12161700

$52.90

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Quality Segment

grade

BioPerformance Certified, Molecular Biology

form

liquid

storage condition

dry at room temperature (tightly closed, well-ventilated place.)

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)

pH

7.0

useful pH range

7.0-9.0

absorption

≤0.05 at 290 at 40%

storage temp.

room temp

General description

A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.
Tris(hydroxymethyl)aminomethane (Tris) is a standard buffer in biological sciences and has a pH buffering range between 7.0 - 9.0. It is applicable in electrophoretic studies and capillary electrochromatography.

Application

Trizma® hydrochloride solution has been used:
  • as a buffer in reverse transcription and DNase I treatment reactions for RNA isolated from Toxoplasma gondii
  • in the preparation of tris- ethylenediaminetetraacetic acid (EDTA) (TE) buffer for bacterial pellet resuspension for plasmid isolation studies
  • for polylactic acid nanoparticle resuspension, storage, and pH-based stability studies

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

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description

pH 7.0, BioPerformance Certified, 1 M, suitable for cell culture

description

pH 7.6, BioPerformance Certified, 1 M, suitable for cell culture

description

pH 9.0, BioPerformance Certified, 1 M, suitable for cell culture

description

pH 8.0, BioPerformance Certified, 2 M, suitable for cell culture

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

grade

Molecular Biology, BioPerformance Certified

grade

Molecular Biology, BioPerformance Certified

grade

Molecular Biology, BioPerformance Certified

grade

Molecular Biology, BioPerformance Certified

concentration

1 M

concentration

1 M

concentration

1 M

concentration

2 M

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

liquid

form

solution

form

solution

form

solution

storage condition

dry at room temperature (tightly closed, well-ventilated place.)

storage condition

-

storage condition

-

storage condition

-

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Storage Class

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Questions

  1. What are the storage conditions for this product?

    1 answer

    1. This product is to be stored in room temperature. Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.

      For more safety and handling information, please view the product Safety Data Sheet:
      https://www.sigmaaldrich.com/sds/sigma/t1819

      Helpful?

Reviews

1 of 1 reviewers took part in a promotion

Active Filters

    • Review 1
    • Votes 0
    4 out of 5 stars.

    NaOCl Sodium Hypochlorite

    All good, however, the delivery time was longer than expected.

    Helpful?