This product has not been in-house tested in a buffer containing NP-40. However, this product is cited in applications within the following article: Yinuo Yang, Shouping Zhang, Jindong Guan, Yuhui Jiang, Jing Zhang, Lan Luo, Cheng Sun, "SIRT1 attenuates neuroinflammation by deacetylating HSPA4 in a mouse model of Parkinson's disease," Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Volume 1868, Issue 5, 2022, 166365.
T1952
Trichostatin A, Ready Made Solution
5 mM in DMSO, from Streptomyces sp.
Synonym(s):
TSA, [R-(E,E)]-7-[4-(Dimethylamino)phenyl]-N-hydroxy-4,6-dimethyl-7-oxo-2,4-heptadienamide
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|---|---|
200 μL | Available to ship TODAYfromMILWAUKEE | $99.70 |
About This Item
Empirical Formula (Hill Notation):
C17H22N2O3
CAS Number:
Molecular Weight:
302.37
UNSPSC Code:
12352202
NACRES:
NA.32
Form:
DMSO solution
Assay:
≥98% (HPLC)
Biological source:
Streptomyces sp.
biological source
Streptomyces sp.
Quality Segment
assay
≥98% (HPLC)
form
DMSO solution
concentration
5 mM in DMSO
technique(s)
cell culture | mammalian: suitable
antibiotic activity spectrum
fungi, neoplastics
mode of action
enzyme | inhibits
shipped in
dry ice
storage temp.
−20°C
SMILES string
N(O)C(=O)\C=C\C(=C\C(C)C(=O)c1ccc(cc1)N(C)C)\C
InChI
1S/C17H22N2O3/c1-12(5-10-16(20)18-22)11-13(2)17(21)14-6-8-15(9-7-14)19(3)4/h5-11,13,22H,1-4H3,(H,18,20)/b10-5+,12-11+
InChI key
RTKIYFITIVXBLE-WKWSCTOISA-N
General description
Trichostatin A is a compound of primary hydroxamic acid.
Application
Trichostatin A, ready-made solution has been used:
- as a histone deacetylase inhibitor to study its effect on transcriptome changes by stem cell testing
- to inhibit histone deacetylase (HDAC) class I, II, or III in primary pituitary cell cultures and to investigate insulin control of endogenous human growth hormone gene (hGH)
- to treat cells for the HDAC inhibition experiments
Biochem/physiol Actions
Trichostatin A (TSA) is a Streptomyces metabolite, which specifically inhibits mammalian histone deacetylase at a nanomolar concentration and causes accumulation of highly acetylated histone molecules in mammalian cells. For that reason, trichostatin A is a tool to study the consequences of histone acetylation in vivo. Trichostatin A induces cell differentiation, cell cycle arrest, reversal of transformed cells morphology, and apoptosis and is able to modulate transcription. TSA has been used to establish a new cloning technique, which increases the success rates for mouse cloning.
Trichostatin A is a Streptomyces metabolite, which specifically inhibits mammalian histone deacetylase.
1 of 1
This Item | |||
|---|---|---|---|
| description 5 mM in DMSO, from Streptomyces sp. | description A potent and reversible, cell-permeable inhibitor of histone deacetylase. | description ≥98% (HPLC), from Streptomyces sp. | description 1 mg/mL in DMSO |
| assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay - |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) - |
| biological source Streptomyces sp. | biological source - | biological source Streptomyces sp. | biological source - |
| concentration 5 mM in DMSO | concentration - | concentration - | concentration 1 mg/mL in DMSO |
| form DMSO solution | form lyophilized solid | form powder | form DMSO solution |
| shipped in dry ice | shipped in ambient | shipped in - | shipped in dry ice |
WGK
WGK 1
Storage Class
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
Can these products be added to an NP-40–based lysis buffer for use in mouse tissue lysis?
1 answer-
Helpful?
-
-
How long is this reagent good for when stored at -20? Do we need to avoid freeze-thaw cycles?
1 answer-
This product may be stored at -20°C in aliquots at least until the recommended retest date published in the Certificate of Analysis. Avoid repeated freeze/thaw cycles.
Helpful?
-