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Anti-TUBA1B (alpha-tubulin ubiquitous) Antibody

Name: Anti-TUBA1B (alpha-tubulin ubiquitous) Antibody
Brand: SIGMA
Price: 601 USD
Availability: InStock

rabbit polyclonal

Synonym(s):

Anti-K-ALPHA-1

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Size/SKU	Availability	Price
0.2 mL	Check Cart for Availability	$601.00
1 mL	Check Cart for Availability	$1,510.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00145921

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IF, WB

Citations:

$601.00

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Properties

Product Name

Anti-Tubulin antibody produced in rabbit, whole antiserum

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

chicken, mouse

technique(s)

indirect immunofluorescence: 1:80 using cultured fibroblasts, western blot: 1:200 using chicken gizzard extract or mouse brain extract

UniProt accession no.

P68363

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Tuba1b(22143), Tubb2a(22151), Tubb2b(73710), Tubb2c(227613)

Description

General description

Microtubules are composed of tubulin and several additional proteins designated microtubule associated proteins (MAPs). Tubulin is a heterodimer which consists of an α-chain and a β-chain, both of which have a molecular weight of approximately 55,000. Microtubules form an elaborate cytoplasmic network during interphase and are reorganized into the mitotic spindle during cell division.

Tubulin is an extremely conserved and negatively charged protein. It is expressed in all eukaryotic cells, but mainly in brains.

Immunogen

microtubule proteins from brains of 13 day old chicken embryos

Application

Anti-Tubulin antibody produced in rabbit has been used in immunoblotting and indirect immunofluorescence.

Biochem/physiol Actions

αβ-tubulin heterodimers helps in formation of microtubules. Tubulin dimers exhibits GTPase activity, assembly and disassembly properties. It facilitates the construction of the flagellar apparatus.

The product stains tubulin in cultured chicken fibroblasts or 3T3 mouse fibroblasts. Additional bands may be stained in immunoblotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	T7451	T6793	T5201
Sigma-Aldrich T3526 Anti-Tubulin antibody produced in rabbit	Sigma-Aldrich T7451 Anti-Acetylated Tubulin antibody, Mouse monoclonal	Sigma-Aldrich T6793 Monoclonal Anti-Tubulin, Acetylated antibody produced in mouse	Sigma-Aldrich T5201 Anti-β-Tubulin antibody, Mouse monoclonal
description whole antiserum	description clone 6-11B-1, purified from hybridoma cell culture	description clone 6-11B-1, ascites fluid	description clone TUB 2.1, purified from hybridoma cell culture
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
biological source rabbit	biological source mouse	biological source mouse	biological source mouse
antibody form whole antiserum	antibody form purified immunoglobulin	antibody form ascites fluid	antibody form purified immunoglobulin
technique(s) western blot: 1:200 using chicken gizzard extract or mouse brain extract, indirect immunofluorescence: 1:80 using cultured fibroblasts	technique(s) radioimmunoassay: suitable, western blot: 0.03-0.06 μg/mL using total rat brain extract, electron microscopy: suitable, dot blot: suitable, immunohistochemistry: suitable	technique(s) electron microscopy: suitable, indirect ELISA: suitable, radioimmunoassay: suitable, immunocytochemistry: suitable, western blot: 1:2,000 using a bovine or rat brain preparation, dot blot: suitable	technique(s) indirect immunofluorescence: 10-20 μg/mL using cultured chicken fibroblasts, western blot: 2-4 μg/mL using chicken fibroblasts cell extract, microarray: suitable, immunoelectrophoresis: suitable, immunohistochemistry: suitable
Gene Information mouse ... Tuba1b(22143), Tubb2a(22151), Tubb2b(73710), Tubb2c(227613)	Gene Information human ... TUBA4A(7277) mouse ... Tuba1a(22142) rat ... Tuba1a(64158)	Gene Information human ... TUBA4A(7277) mouse ... Tuba1a(22142) rat ... Tuba1a(64158)	Gene Information human ... TUBB(203068), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383) mouse ... Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb3(22152) rat ... Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554)

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Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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