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Anti-γ-Tubulin Antibody

Name: Anti-γ-Tubulin Antibody
Brand: SIGMA
Price: 192 USD
Availability: InStock

Enhanced Validation

rabbit polyclonal

Synonym(s):

Anti-γ-Tubulin

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Size/SKU	Availability	Price
25 μL	Available to ship TODAYfromMILWAUKEE	$192.00
0.2 mL	Available to ship TODAYfromMILWAUKEE	$598.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, IF, WB

Citations:

177

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Properties

Product Name

Anti-γ-Tubulin antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 48 kDa

species reactivity

human, chicken

packaging

antibody small pack of 25 μL

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

indirect immunofluorescence: 1:5,000 using methanol/acetone-fixed chicken fibroblasts, microarray: suitable, western blot: 1:1,000 using cultured A431 whole cell extracts

UniProt accession no.

P23258

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBG1(7283), TUBG2(27175)

Description

General description

γ-Tubulin (48kDa) is a ubiquitous and highly conserved protein within the microtubule organizing centers (MTOCs) in eukaryotic cells. It is related to α- and β-tubulin and is a member of the tubulin superfamily of proteins. γ-Tubulin abundance is less than 1% of the level of either α- or β-tubulin. γ-Tubulin shares approximately 28-32% identity with α-tubulin from various organisms and 32-36% identity with β-tubulins. Some regions are highly conserved among α-, β- and γ-tubulins.

Immunogen

synthetic peptide corresponding to the N-terminal region of human γ-tubulin (amino acids 38-53, with C-terminally added lysine) conjugated to KLH. This sequence is specific for γ-tubulin and not found in other members of the tubulin family such as α− β−, δ and ε− tubulins. This sequence is identical in mouse and rat γ-tubulin and highly conserved among species (Drosophila, Aspergillus, and yeast γ-tubulin).

Application

Anti-γ-Tubulin antibody produced in rabbit has been used in

immunofluorescence analysis
western blotting
immunohistochemistry

Anti-γ-Tubulin polyclonal antibody is suitable for immunoblotting of materials such as A431 cultured cells whole extract (g-tubulin, 48 kDa), and immunocytochemical staining of cells such as cultured chicken fibroblast cells.

Biochem/physiol Actions

γ-Tubulin binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome. γ-Tubulin is not a member of microtubule like α- and β-tubulin. It functions as the microtubule nucleator at the microtubule organizing centre (MTOC). It binds to the β-tubulin half of the tubulin molecule, thus establishing the polarity of a microtubule, leaving the α-tubulin half exposed at the plus end.

Anti-gamma-Tubulin recognizes an epitope located in the N-terminal region of gamma-tubulin (amino acids 38-53). Staining of gamma-tubulin (48 kDa) by immunoblotting is specifically inhibited with gamma-tubulin peptide (human, amino acids 38-53 with C-terminally added lysine).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	T3320	T5293	T6074
$Anti-γ-Tubulin antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich T3559 Anti-γ-Tubulin antibody produced in rabbit	$Anti-γ-Tubulin (AK-15) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich T3320 Anti-γ-Tubulin (AK-15) antibody produced in rabbit	Sigma-Aldrich T5293 Monoclonal Anti-β-Tubulin antibody produced in mouse	Sigma-Aldrich T6074 Anti-α-Tubulin antibody, Mouse monoclonal
description IgG fraction of antiserum, buffered aqueous solution	description IgG fraction of antiserum, buffered aqueous solution	description clone 2-28-33, ascites fluid	description clone B-5-1-2, purified from hybridoma cell culture
biological source rabbit	biological source rabbit	biological source mouse	biological source mouse
antibody form IgG fraction of antiserum	antibody form IgG fraction of antiserum	antibody form ascites fluid	antibody form purified from hybridoma cell culture
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
Gene Information human ... TUBG1(7283), TUBG2(27175)	Gene Information human ... TUBG1(7283), TUBG2(27175) rat ... Tubg1(252921), Tubg2(680991)	Gene Information human ... TUBB1(81027), TUBB2A(7280), TUBB2C(10383) rat ... Tubb2(29212), Tubb2c(296554)	Gene Information human ... TUBA4A(7277) mouse ... Tuba1a(22142) rat ... Tuba1a(64158)
UniProt accession no. P23258	UniProt accession no. P23258	UniProt accession no. Q9H4B7	UniProt accession no. P68366

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c