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T3809

TRI Reagent®

BD, For processing whole blood, plasma, or serum.

Synonym(s):

TRI Reagent® RNA Isolation Reagent

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Size/SKUAvailabilityPrice
25 mL

Available to ship onOctober 07, 2026fromMILWAUKEE

$108.00
100 mL

Available to ship onOctober 07, 2026fromMILWAUKEE

$338.00
200 mL

Available to ship onOctober 07, 2026fromMILWAUKEE

$600.00

About This Item

UNSPSC Code:
12352200
NACRES:
NA.52
MDL number:
MFCD00213058

$108.00

Available to ship onOctober 07, 2026Details

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Quality Segment

usage

0.75 mL sufficient for 0.25 mL blood derivatives

General description

TRI Reagent BD is a quick and convenient reagent for use in the simultaneous isolation of RNA, DNA and protein from serum, plasma or whole blood. A convenient single-step liquid phase separation results in the simultaneous isolation of RNA, DNA and protein. This procedure is an adaptation of the single-step method reported by Chomczynski and Sacchi for total RNA isolation, and permits fast and efficient processing of blood derivatives.

TRI REAGENT BD performs well with large or small sample volumes, and many samples can be simultaneously extracted. TRI Reagent BD is a mixture of guanidine thiocyanate and phenol in a mono-phase solution. When a sample of blood derivatives is lysed with it, and chloroform or 1-bromo-3-chloropropane is added, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing DNA and an organic phase containing proteins. Each component can then be isolated after separating the phases. 0.75 ml of TRI REAGENT BD processes 0.25 ml of blood derivatives.

This is one of the most effective methods for isolating total RNA and can be completed in only 1 hour starting with fresh cells. The procedure is very effective for isolating RNA molecules of all types from 0.1 to 15 kb in length. The resulting RNA is intact with little or no contaminating DNA or protein.
TRI Reagent® is a monophasic solution of phenol and guanidinium isothiocyanate.

Application

TRI Reagent® has been used for the isolation of RNA.
TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. The RNA isolation method based on this reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.
The resulting RNA can be used for Northern blots, mRNA isolation, in vitro translation, RNase protection assays, cloning and PCR.

Features and Benefits

  • Easily scalable RNA isolation
  • Works with many sources: human, plant, yeast, bacterial, or viral
  • Better yields than traditional guanidine thiocyanate/cesium chloride methods

Legal Information

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

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Sigma-Aldrich

T9424

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description

BD, For processing whole blood, plasma, or serum.

description

LS, For processing fluid samples such as cell suspensions, CSF, and amniotic fluid.

description

-

description

For processing tissues, cells cultured in monolayer or cell pellets

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

200

usage

0.75 mL sufficient for 0.25 mL blood derivatives

usage

0.75 mL sufficient for 0.25 mL fluid samples

usage

 mL sufficient for 107 cells,  mL sufficient for 100 mg tissue

usage

 mL sufficient for 107 cells,  mL sufficient for 100 mg tissue (or)

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Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Target Organs

Nervous system,Kidney,Liver,Skin

supp_hazards

EUH032

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point (°F)

174.2 °F - closed cup

Flash Point (°C)

79 °C - closed cup

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Questions

  1. When using Tri Reagent BD, do we need to add EDTA to blood samples to prevent coagulation?

    1 answer

    1. The Product Information available under More Documents or at this link (https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/436/845/t3809bul.pdf) provides the following instructions:

      I. Sample Preparation:

      1A. Serum: Add 0.25 ml of serum to 0.75 ml of TRI Reagent BD. Close the tube and shake the solution by hand or vortex, ensuring that mixing is thorough.

      1B. Whole Blood or Plasma: Add 0.2 ml of whole blood or plasma to 0.75 ml of TRI Reagent BD supplemented with 20 ml of 5 N acetic acid per 0.2 ml of whole blood or plasma. Close the tube and shake the solution by hand or vortex, ensuring that mixing is thorough.

      It is possible to use whole blood (blood that has been allowed to clot), serum, or plasma. Blood that has clotted would be referred to as a clot plus serum or simply as clotted blood. If you are freshly drawing the blood and adding immediately to the Tri Reagent, an anticoagulant would not be required. For serum samples, the blood is simply allowed to clot. The clot is spun down and serum is collected above the clot. If plasma is used, this indicates the blood is first drawn into either a tube containing an anticoagulant or drawn with a syringe and then transferred to a tube containing an anticoagulant. The blood is then centrifuged and the plasma is removed before adding to the Tri Reagent. We would not recommend allowing the blood to clot and then trying to add the clotted blood to the Tri Reagent.

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