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T4184

Thrombopoietin from mouse

recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture, >97% (SDS-PAGE)

Synonym(s):

TPO

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5 μg
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$665.00

About This Item

CAS Number:
9014-42-0
UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
MFCD01095336
Form:
lyophilized powder
Assay:
>97% (SDS-PAGE)
Biological source:
mouse
Recombinant:
expressed in NSO cells
Mol wt:
~75 kDa by SDS-PAGE (as a result of glycosylation), predicted mol wt 35 kDa

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biological source

mouse

Quality Segment

200

recombinant

expressed in NSO cells

assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

2.1 ng/mL EC50

mol wt

~75 kDa by SDS-PAGE (as a result of glycosylation), predicted mol wt 35 kDa

packaging

pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

P40226

storage temp.

−20°C

Gene Information

mouse ... Thpo(21832)

Application

Thrombopoietin from mouse can be used in a genetic screening test for examining the role of somatic activating mutations in EPOR, MPL, or GCSFR genes in activating myeloproliferative disorders (MPD) in JAK2V617F-negative MPD patients.

Biochem/physiol Actions

Thrombopoietin from mouse is an amino acid peptide that plays a pivotal role in megakaryocyte proliferation and differentiation. It also serves as a growth factor for liver endothelial cells in mouse.
TPO is a ligand for the c-mpl proto-oncogene (Mpl-L), a megakaryocyte colony stimulating factor (Meg-CSF), and a primary regulatory factor for megakaryocytopoiesis and thrombopoiesis. TPO is a humoral glycoprotein that stimulates growth and maturation of megakaryocytes and megakaryocytic colonies from bone marrow cultures. TPO binds and activates a 68-78 kDa glycoprotein receptor belonging to the GH family of cytokine receptors, a family that includes receptors to growth hormone (GH), erythropoietin (EPO) and prolactin (PRL). TPO receptors are found on megakaryocytes and their precursors and on platelets. The mature form of TPO is a highly conserved, showing approximately 70% sequence homology among various mammals.
Thrombopoeitin (TPO), also known as the ligand for the c-mpl proto-oncogene (Mpl-L) and as megakaryocyte colony stimulating factor (Meg-CSF), is a primary regulatory factor for megakaryocytopoiesis and thrombopoiesis. Produced mainly by hepatocytes but also by kidney cells, TPO is a humoral glycoprotein that stimulates growth and maturation of megakaryocytes and megakaryocytic colonies from bone marrow cultures. TPO binds and activates an 68-78 kDa glycoprotein receptor belonging to the GH family of cytokine receptors, a family that includes receptors to growth hormone (GH), erythropoietin (EPO) and prolactin (PRL). Like GH and EPO, TPO may bind to its receptor at two distinct sites, initiating receptor dimerization and activation. Analysis of mRNA indicates also the existence of a novel truncated and potentially soluble form of TPO receptor. The viral oncogene v-mlp of the myeloproliferative leukemia virus (MPLV) contains the gene sequence for the entire cytoplasmic and transmembrane domains and a portion of the extracellular domain of c-mlp (TPO receptor). TPO receptors are found on megakaryocytes and their precursors and on platelets. The mature form of TPO is a highly conserved glycoprotein, showing approximately 70% sequence homology among various mammals. Human TPO contains 332 amino acids (mouse 335) including two internal disulfide bonds and 6 N-linked glycosylation sites (mouse 7). The N-terminal 153 amino acid region of TPO shows structural and amino acid sequence homology to erythropoietin and a pair of arginines (potential cleavage site) separating it from the highly glycosylated carboxyterminal domain.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.25 mg bovine serum albumin

Analysis Note

The biological activity was measured in a cell proliferation assay using MO7e cells.

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Thrombopoietin from mouse recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture, >97% (SDS-PAGE)

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Insulin-Like Growth Factor Binding Protein-2 human recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture, ≥95% (SDS-PAGE)

Sigma-Aldrich

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Insulin-Like Growth Factor Binding Protein-2 human

description

recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture, >97% (SDS-PAGE)

description

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

description

lyophilized powder, recombinant, expressed in baculovirus infected Sf21 cells, suitable for cell culture

description

recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture, ≥95% (SDS-PAGE)

assay

>97% (SDS-PAGE)

assay

≥98% (SDS-PAGE), ≥98% (HPLC)

assay

>97% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

recombinant

expressed in NSO cells

recombinant

expressed in E. coli

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in NSO cells

biological source

mouse

biological source

mouse

biological source

mouse

biological source

human

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

lyophilized powder

form

lyophilized

form

lyophilized powder

form

lyophilized powder

mol wt

predicted mol wt 35 kDa, ~75 kDa by SDS-PAGE (as a result of glycosylation)

mol wt

18.7 kDa

mol wt

~38 kDa by SDS-PAGE

mol wt

calculated mol wt ~31 kDa, ~36 kDa by SDS-PAGE

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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