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Anti-β-Tubulin antibody, Mouse monoclonal

Name: Anti-β-Tubulin antibody, Mouse monoclonal
Brand: SIGMA

clone TUB 2.1, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-β-Tubulin

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

TUB 2.1, monoclonal

Application:

ARR, IEP, IF, IHC, WB

Citations:

278

Pricing and availability is not currently available.

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

TUB 2.1, monoclonal

form

buffered aqueous solution

mol wt

antigen 55 kDa

species reactivity

human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster

packaging

antibody small pack of 25 μL

concentration

~2 mg/mL

technique(s)

indirect immunofluorescence: 10-20 μg/mL using cultured chicken fibroblasts, microarray: suitable, immunoelectrophoresis: suitable, immunohistochemistry: suitable, western blot: 2-4 μg/mL using chicken fibroblasts cell extract

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Description

General description

β-Tubulin belongs to the subfamily of tubulin, which is the major building block of microtubules. β-Tubulin has a molecular weight of 55kDa. β-Tubulin structure is characterized with core of two β-sheets enclosed by α-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site, and a C-terminal domain that contains the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (βI (class I), βI (class II), βIII (class III), βIVa (class IVa), βIVb (class IVb), βV (class V), and βVI (class VI)).

Monoclonal Anti-β-Tubulin (mouse IgG1 isotype) is derived from the hybridoma TUB 2.1 produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified rat brain tubulin.

Immunogen

tubulin from rat brain.

Application

Monoclonal Anti-β-Tubulin antibody produced in mouse has also been used in

2-dimensional electrophoresis
sperm motility
immunohistochemistry,
immunofluorescence[1]
immunoblotting[1]
immunocytochemistry[2]

Monoclonal Anti-β-Tubulin antibody produced in mouse has been used in western blotting.

Biochem/physiol Actions

Mutation in the gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. B2702 peptide binds to β-tubulin and inhibits NK cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. β-1 tubulin also known as class VI or TUBB1, plays a vital role in platelet production, and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).

The antibody recognizes all five isoforms of β-tubulin (β1-β5). It reacts with the β-Lc and β-Sc fragments in the carboxy-terminal part of β−tubulin in immunoblotting. The antibody may be used to localize β−tubulin in cultured cells or tissue sections.

Physical form

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB4200715	SAB4200792	T8578
Sigma-Aldrich T5201 Anti-β-Tubulin antibody, Mouse monoclonal	Sigma-Aldrich SAB4200715 Anti-β-tubulin antibody, Mouse monoclonal	Sigma-Aldrich SAB4200792 Anti-β-Tubulin IV antibody, Mouse monoclonal	Sigma-Aldrich T8578 Anti-β-Tubulin III (neuronal) antibody, Mouse monoclonal
description clone TUB 2.1, purified from hybridoma cell culture	description clone 2-28-33, purified from hybridoma cell culture	description clone ONS.1A6, purified from hybridoma cell culture	description ~1.0 mg/mL, clone 2G10, purified from hybridoma cell culture
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture
biological source mouse	biological source mouse	biological source mouse	biological source mouse
clone TUB 2.1, monoclonal	clone 2-28-33, monoclonal	clone ONS.1A6, monoclonal	clone 2G10, monoclonal
species reactivity human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster	species reactivity dog, chicken, rat, bovine, monkey, human, mouse	species reactivity chicken, bovine, rat, mouse, human	species reactivity human, mouse, rat
Gene Information human ... TUBB(203068), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383) mouse ... Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb3(22152) rat ... Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554)	Gene Information human ... TUBB(203068) mouse ... Tubb3(22152)	Gene Information human ... TUBB(203068) mouse ... Tubb3(22152)	Gene Information human ... TUBB3(10381) mouse ... Tubb3(22152) rat ... Tubb3(246118)

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Storage Class

12 - Non Combustible Liquids

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