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Tricine

Name: Tricine
Brand: SIGMA
Price: 90.3 USD
Availability: InStock

BioPerformance Certified, suitable for cell culture, ≥99% (titration)

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Synonym(s):

2-[[1,3-Dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid, N-[Tris(hydroxymethyl)methyl]glycine, N-[Tris(hydroxymethyl)methyl]glycine

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Size/SKU	Availability	Price
25 g	Available to ship TODAY	$90.30
100 g	Available to ship TODAY	$161.00
500 g	Available to ship TODAY	$605.00
1 kg	Available to ship TODAY	$871.00

About This Item

Linear Formula:

(HOCH₂)₃CNHCH₂CO₂H

CAS Number:

5704-04-1

Molecular Weight:

179.17

UNSPSC Code:

12161700

NACRES:

NA.25

PubChem Substance ID:

24900318

EC Number:

227-193-6

Beilstein/REAXYS Number:

1937804

MDL number:

MFCD00004277

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Properties

Quality Segment

400

grade

BioPerformance Certified

assay

≥99% (titration)

form

crystalline powder

storage condition

dry at room temperature

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin and total aerobic microbial count, tested

color

white

useful pH range

7.4-8.8

pKa (25 °C)

8.1

mp

187 °C

cation traces

heavy metals (as Pb): ≤5 ppm

absorption

≤0.02 at 290 at 20%

application(s)

diagnostic assay manufacturing
general analytical
life science and biopharma
sample preparation

SMILES string

OCC(CO)(CO)NCC(O)=O

InChI

1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)

InChI key

SEQKRHFRPICQDD-UHFFFAOYSA-N

Description

General description

Tricine is a zwitterionic biological buffer commonly used in enzyme assays, electrophoresis, and cell culture research. It has a higher negative charge compared to glycine, which enables it to migrate more rapidly. Tricine′s high ionic strength has the ability to cause more ion movement and less protein movement, resulting in the separation of low molecular weight proteins in lower percent acrylamide gels. It has been observed that Tricine is efficient in the separation of proteins ranging from 1 to 100 kDa through electrophoresis. According to a study, the 25 mmol/L Tricine buffer proved to be the most effective among ten other buffers tested for ATP assays using firefly luciferase. Further, Tricine is also used in capillary zone electrophoresis, high performance liquid chromatography and ion exchange chromatography.

Application

Buffer component for separation of low molecular weight peptides.

Tricine serves as a versatile buffering agent suitable for separating low molecular weight proteins in lower percent acrylamide gels, has demonstrated efficacy in ATP assays employing firefly luciferase, and has shown potential in scavenging hydroxyl radicals during the investigation of radiation-induced membrane damage. Additionally, it has played a role in isolating and purifying ribosomes engaged in the translation of specific mRNA and containing specific peptidyl-tRNA molecules. Tricine has also been employed in the synthesis of RNA oligonucleotides and the fabrication of high-density DNA microarrays, enabling the exploration of mechanisms underlying nucleic acid·ligand interactions and library preparation through photolithography.

Features and Benefits

Suitable as a Buffer component, for Electrophoresis and Protein separation
Effective Buffering from pH 7.4-8.8 (25 °C) with a pKa of 8.1 (25 °C)
Tested for Endotoxins and Total Aerobic Microbial Count
Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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This Item	T17917	T0377	RDD024
Sigma-Aldrich T5816 Tricine	Sigma-Aldrich T17917 Tricine	Sigma-Aldrich T0377 Tricine	Sigma-Aldrich RDD024 Tricine
description BioPerformance Certified, suitable for cell culture, ≥99% (titration)	description ≥99% (titration), reagent grade, crystalline	description ≥99% (titration)	description anhydrous, free-flowing, Redi-Dri^™, ≥99%
technique(s) cell culture \| mammalian: suitable	technique(s) -	technique(s) SDS-PAGE: suitable	technique(s) -
assay ≥99% (titration)	assay ≥99% (titration)	assay ≥99% (titration)	assay ≥99%
grade BioPerformance Certified	grade reagent grade	grade -	grade anhydrous
Quality Level 400	Quality Level 200	Quality Level 400	Quality Level 100
application(s) diagnostic assay manufacturing general analytical life science and biopharma sample preparation	application(s) -	application(s) diagnostic assay manufacturing	application(s) -
form crystalline powder	form crystalline	form crystalline powder	form powder

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Storage Class

13 - Non Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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