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Anti-Rabbit IgG (whole molecule)–TRITC antibody produced in goat

Name: Anti-Rabbit IgG (whole molecule)–TRITC antibody produced in goat
Brand: SIGMA
Price: 304 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Goat Anti-Rabbit IgG (whole molecule)–Tetramethylrhodamine isothiocyanate

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1 mL	Available to ship TODAYfromMILWAUKEE	$304.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162790

Conjugate:

TRITC conjugate

Clone:

polyclonal

Application:

IF (d), IHC (p)

Citations:

103

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Properties

biological source

goat

Quality Segment

200

conjugate

TRITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

contains

≥15 mM sodium azide as preservative

storage condition

protect from light

technique(s)

direct immunofluorescence: 1:400, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Binds all rabbit Igs.

IgG consists of four isotypes IgG1, IgG2, IgG3 and IgG4. It contains four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which is linked together by inter-chain disulfide bonds.

Application

Conjugation of TRITC to Rabbit IgG (whole molecule) antibody enables the use in immunofluorescent techniques.

Anti-Rabbit IgG (whole molecule)−TRITC antibody produced in goat has been used in immunocytochemistry.

Biochem/physiol Actions

IgG activates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody-dependent cell-mediated cytotoxicity (ADCC) and phagocytosis. It might be associated with allergy. IgG mediates transplacental transport and participates in the secondary immune response. It has the longest serum half life.

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4.

Preparation Note

Adsorbed to reduce background staining with human samples.

Other Notes

Antibody adsorbed with human IgG.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	F9887	F0382	A9169
Sigma-Aldrich T6778 Anti-Rabbit IgG (whole molecule)–TRITC antibody produced in goat	Sigma-Aldrich F9887 Anti-Rabbit IgG (whole molecule)–FITC antibody produced in goat	Sigma-Aldrich F0382 Anti-Rabbit IgG (whole molecule)–FITC antibody produced in goat	$Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich A9169 Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat
description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description IgG fraction of antiserum, buffered aqueous solution
biological source goat	biological source goat	biological source goat	biological source goat
conjugate TRITC conjugate	conjugate FITC conjugate	conjugate FITC conjugate	conjugate peroxidase conjugate
technique(s) direct immunofluorescence: 1:400, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160, direct immunofluorescence: 1:160	technique(s) indirect immunofluorescence: 1:80	technique(s) western blot (chemiluminescent): 1:80,000-1:160,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per well), direct ELISA: 1:50,000 using using 5 μg/ml of rabbit IgG for coating and OPD substrate, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form IgG fraction of antiserum
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C
form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution

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Storage Class

10 - Combustible liquids

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