Yes, this product may be reconstituted in the vial using sterile water to a concentration of 0.1 mg/mL._x000D_
The lyophilized product should be stored at –20 °C. The reconstituted product can be stored in working aliquots at –20°C for at least 1 year. Repeated freezing and thawing, or storage in frost-free freezers is not recommended. Please see the link below to review additional information available in the product datasheet:_x000D_
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/758/965/t7539dat.pdf
T7539
Tumor Necrosis Factor-α from mouse
≥95%, recombinant, expressed in E. coli, powder, suitable for cell culture
Synonym(s):
mTNF-α, TNF-α
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| Size/SKU | Availability | Price |
|---|---|---|
10 μg | Check Cart for Availability | $246.00 |
50 μg | Check Cart for Availability | $616.00 |
About This Item
Biological source:
mouse
Recombinant:
expressed in E. coli
Assay:
≥95%
Form:
powder
Mol wt:
17 kDa
Impurities:
≤1 EU/μg protein endotoxin
Product Name
Tumor Necrosis Factor-α from mouse, TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture
biological source
mouse
Quality Segment
recombinant
expressed in E. coli
assay
≥95%
form
powder
mol wt
17 kDa
packaging
pkg of 10 μg, pkg of 50 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μg protein endotoxin
color
white
solubility
water: soluble
suitability
suitable for ((EC50) < 0.500 ng/mL)
UniProt accession no.
storage temp.
−20°C
Application
Tumor Necrosis Factor-α from mouse, a potent proinflammatory cytokine has been used:
- to activate MS1 endothelium cells
- to induce lipolysis in adipocytes
- to study its effect on liganded and unliganded glucocorticoid receptor (GR) in interleukin (IL)-6 and IL-8 gene regulation
Biochem/physiol Actions
Tumor necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is glycosylated.
Tumor necrosis factor-α, also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is cytotoxic for many transformed cells, but in normal diploid cells, it stimulates proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and induces production of several other cytokines.
Physical form
Lyophilized from 10 mM sodium phosphate, pH 7.5
Other Notes
One unit is the amount required to induce half-maximal cytolysis of L929 cells.
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This Item | |||
|---|---|---|---|
| description TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture | description TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture | description Xeno-free, recombinant, expressed in HEK 293 cells, suitable for cell culture | description affinity isolated antibody, lyophilized powder |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in HEK 293 cells | recombinant - |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) flow cytometry: 5-25 μg/mL, indirect immunofluorescence: 15 μg/mL using mouse cells and tissues, neutralization: suitable, capture ELISA: suitable, western blot: 0.1-0.2 μg/mL |
| assay ≥95% | assay ≥98% (SDS-PAGE and HPLC) | assay ≥95% (SDS-PAGE) | assay - |
| biological source mouse | biological source rat | biological source human | biological source goat |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| form powder | form powder | form lyophilized powder | form lyophilized powder |
Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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What is the stability of TNF when dissolved in water? Can dissolved TNF be stored in the freezer? If so, how long is the compound stable?
1 answer-
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What are the units/mg of Product T7539, Tumor Necrosis Factor-α mouse?
1 answer-
1 mg of rmTNF-alpha = 2.7 × 10^8 WHO units.
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