For western blotting we use 1 % Non-fat dry milk (NFDM) for diluting the antibody before use.
T8578
Anti-β-Tubulin III Antibody
mouse monoclonal, 2G10
Synonym(s):
Anti-β-Tubulin Class III, Anti-Neuron-specifc class III β-tubulin, Anti-TUBB4, Anti-Tubb3, Anti-Tubulin β-4, Anti-Tubulin β-III, Anti-Tubulin, β−3
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| Size/SKU | Availability | Price |
|---|---|---|
25 μL | Check Cart for Availability | $208.00 |
100 μL | Check Cart for Availability | $601.00 |
200 μL | Check Cart for Availability | $750.00 |
About This Item
Conjugate:
unconjugated
Clone:
2G10, monoclonal
Application:
ICC, IHC, IP, WB
Citations:
258
Product Name
Anti-β-Tubulin III (neuronal) antibody, Mouse monoclonal, ~1.0 mg/mL, clone 2G10, purified from hybridoma cell culture
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
2G10, monoclonal
form
buffered aqueous solution
mol wt
antigen ~50 kDa
species reactivity
human, mouse, rat
packaging
antibody small pack of 25 μL
concentration
~1.0 mg/mL
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: 0.25-0.5 μg/mL using PC12 cell extract, immunohistochemistry: suitable
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TUBB3(10381)
mouse ... Tubb3(22152)
rat ... Tubb3(246118)
General description
Tubulin β 3 class III (TUBB3) also known as β-Tubulin III, is encoded by the gene mapped to human chromosome 16q24.3. TUBB3 protein expression is restricted to neurons.
Application
Biochem/physiol Actions
Tubulin β 3 class III (TUBB3) plays a vital role in nervous system development and axon guidance. Mutation of the gene encoding protein leads to the ocular motility disorder, congenital fibrosis of the extraocular muscle type 3 (CFEOM3), and various neurological syndromes. Altered expression of the protein affects microtubule dynamics and microtubule-kinesin interactions. Increased expression of TUBB3 is observed in tumor tissues. Decreased expression of TUBB3 can be considered as an important marker for poor prognosis of cutaneous malignant melanoma. TUBB3 is implicated in the suppression of invasive growth of tumor tissue;therefore, it is considered to be a potential target for development of antitumor drugs.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| description ~1.0 mg/mL, clone 2G10, purified from hybridoma cell culture | description clone SDL.3D10, purified from hybridoma cell culture | description affinity isolated antibody, buffered aqueous solution | description ~1 mg/mL, affinity isolated antibody, buffered aqueous solution |
| biological source mouse | biological source mouse | biological source rabbit | biological source rabbit |
| Gene Information human ... TUBB3(10381) | Gene Information human ... TUBB3(10381) | Gene Information human ... TUBB3(10381) | Gene Information human ... TUBB3(10381) |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| antibody form purified from hybridoma cell culture | antibody form purified immunoglobulin | antibody form affinity isolated antibody | antibody form affinity isolated antibody |
| species reactivity human, mouse, rat | species reactivity mouse, rat, human, boar, bovine | species reactivity rat, human, mouse | species reactivity human |
| clone 2G10, monoclonal | clone SDL.3D10, monoclonal | clone polyclonal | clone polyclonal |
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Storage Class
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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can we use Milk 5% to dilute it inside?
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