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Thioredoxin human

Name: Thioredoxin human
Brand: SIGMA
Price: 644 USD
Availability: InStock

≥90% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal histidine tagged), essentially salt-free, lyophilized powder, ≥5 U/mg

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Size/SKU	Availability	Price
0.5 mg	Estimated to ship TODAYfromMILWAUKEE	$644.00

About This Item

CAS Number:

52500-60-4

UNSPSC Code:

12352200

NACRES:

NA.32

MDL number:

MFCD00132423

Form:

essentially salt-free, lyophilized powder

Assay:

≥90% (SDS-PAGE)

Biological source:

human

Recombinant:

expressed in E. coli (N-terminal histidine tagged)

Mol wt:

14 kDa

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Properties

biological source

human

Quality Segment

200

recombinant

expressed in E. coli (N-terminal histidine tagged)

assay

≥90% (SDS-PAGE)

form

essentially salt-free, lyophilized powder

specific activity

≥5 U/mg

mol wt

14 kDa

technique(s)

activity assay: suitable

solubility

water: soluble 0.50—0.60 mg/mL, clear to slightly hazy, colorless to faint yellow or tan

suitability

suitable for molecular biology

UniProt accession no.

P10599

application(s)

cell analysis

storage temp.

−20°C

Gene Information

human ... TXN(7295)

Description

General description

Thioredoxin (TXN) is a 12 kDa ubiquitous protein. The gene is located on human chromosome 9q31.3. TXN is mainly located in the cytoplasm.Thioredoxin contains a redox-active dithiol in the active site. Itexists in several forms, the most prevalent ones being the cytosolic (TRX1) andmitochondrial (TRX2) forms.

Cloned from Jurkat cell cDNA. Identical to the ADF/Trx (GenBank^® Accession Number X77584) and the thioredoxin from human placenta (GenBank^® Accession Number AF085844).

Application

Thioredoxin human can be used for in vitro thioredoxin assay. The product can also be used for assessing Thioredoxin reductase (TRXR) enzyme activity.

Thioredoxin human has been used:

in mitogen-activated protein kinase kinase 4 (MKK4) in vitro kinase assay
to study its role in transcription regulation, RNA processing and nuclear pore function in human cells
in proteomics identification of Trx1 reduction target proteins from the hearts of cardiac specific transgenic (Tg)-Trx1 mice

Biochem/physiol Actions

Human Thioredoxin is a recombinant, N-terminal histidine tagged protein. The product induces proliferation in lymphoid cells, fibroblasts as well as in numerous human solid tumor cell lines.The product also stimulates growth in normal as well as leukemic B cell, modifies the transcriptional activity of AP-1and inhibits the apoptosis signal-regulating kinase 1 (ASK1).Thioredoxin (TRX) plays a role in protecting the cells fromelectrophiles, a cytotoxic agent.

Mediates the reduction of disulfide bonds in proteins.

Thioredoxin (TXN) has disulfide-reducing activity. It acts as a cytokine to reduce inflammation. TXN functions as a scavenger for reactive oxygen intermediates (ROIs), which are produced in various oxidative stress. Thioredoxin induces cellular proliferation to decrease oxidative stress. TXN is a prognostic biomarker for various cancers, such as lung, pancreatic and hepatocellular carcinoma. It contributes to protein reduction, tissue development and redox regulation.

Other Notes

One unit will cause a ΔA₆₅₀ of 1.0 in 1 min at 25 °C in the insulin reduction assay.

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

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This Item	T0910	598101	U5382
Sigma-Aldrich T8690 Thioredoxin human	Sigma-Aldrich T0910 Thioredoxin from Escherichia coli	Sigma-Aldrich 598101 Thioredoxin, Human, Recombinant, E. coli	Sigma-Aldrich U5382 Ubiquitin human
description ≥90% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal histidine tagged), essentially salt-free, lyophilized powder, ≥5 U/mg	description recombinant, expressed in E. coli, essentially salt-free, lyophilized powder, ≥3 units/mg protein	description -	description ≥95% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal FLAG^® tagged), lyophilized powder
recombinant expressed in E. coli (N-terminal histidine tagged)	recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli (N-terminal FLAG^® tagged)
assay ≥90% (SDS-PAGE)	assay ≥90% (SDS-PAGE)	assay ≥90% (SDS-PAGE)	assay ≥95% (SDS-PAGE)
technique(s) activity assay: suitable	technique(s) activity assay: suitable	technique(s) -	technique(s) mass spectrometry (MS): suitable
biological source human	biological source Escherichia coli	biological source -	biological source human
form essentially salt-free, lyophilized powder	form essentially salt-free, lyophilized powder	form lyophilized solid	form lyophilized powder
mol wt 14 kDa	mol wt 11.7 kDa	mol wt -	mol wt 10 kDa

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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