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T8690

Thioredoxin human

≥90% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal histidine tagged), essentially salt-free, lyophilized powder, ≥5 U/mg

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0.5 mg

Estimated to ship TODAYfromMILWAUKEE

$667.00

About This Item

CAS Number:
52500-60-4
UNSPSC Code:
12352200
NACRES:
NA.32
MDL number:
MFCD00132423
Form:
essentially salt-free, lyophilized powder
Assay:
≥90% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli (N-terminal histidine tagged)
Mol wt:
14 kDa

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biological source

human

Quality Segment

recombinant

expressed in E. coli (N-terminal histidine tagged)

assay

≥90% (SDS-PAGE)

form

essentially salt-free, lyophilized powder

specific activity

≥5 U/mg

mol wt

14 kDa

technique(s)

activity assay: suitable

solubility

water: soluble 0.50—0.60 mg/mL, clear to slightly hazy, colorless to faint yellow or tan

suitability

suitable for molecular biology

UniProt accession no.

application(s)

cell analysis

storage temp.

−20°C

Gene Information

human ... TXN(7295)

General description

Thioredoxin (TXN) is a 12 kDa ubiquitous protein. The gene is located on human chromosome 9q31.3. TXN is mainly located in the cytoplasm.Thioredoxin contains a redox-active dithiol in the active site. Itexists in several forms, the most prevalent ones being the cytosolic (TRX1) andmitochondrial (TRX2) forms.
Cloned from Jurkat cell cDNA. Identical to the ADF/Trx (GenBank® Accession Number X77584) and the thioredoxin from human placenta (GenBank® Accession Number AF085844).

Application

Thioredoxin human can be used for in vitro thioredoxin assay. The product can also be used for assessing Thioredoxin reductase (TRXR) enzyme activity.
Thioredoxin human has been used:
  • in mitogen-activated protein kinase kinase 4 (MKK4) in vitro kinase assay
  • to study its role in transcription regulation, RNA processing and nuclear pore function in human cells
  • in proteomics identification of Trx1 reduction target proteins from the hearts of cardiac specific transgenic (Tg)-Trx1 mice

Biochem/physiol Actions

Human Thioredoxin is a recombinant, N-terminal histidine tagged protein. The product induces proliferation in lymphoid cells, fibroblasts as well as in numerous human solid tumor cell lines.The product also stimulates growth in normal as well as leukemic B cell, modifies the transcriptional activity of AP-1and inhibits the apoptosis signal-regulating kinase 1 (ASK1).Thioredoxin (TRX) plays a role in protecting the cells fromelectrophiles, a cytotoxic agent.
Mediates the reduction of disulfide bonds in proteins.
Thioredoxin (TXN) has disulfide-reducing activity. It acts as a cytokine to reduce inflammation. TXN functions as a scavenger for reactive oxygen intermediates (ROIs), which are produced in various oxidative stress. Thioredoxin induces cellular proliferation to decrease oxidative stress. TXN is a prognostic biomarker for various cancers, such as lung, pancreatic and hepatocellular carcinoma. It contributes to protein reduction, tissue development and redox regulation.

Other Notes

One unit will cause a ΔA650 of 1.0 in 1 min at 25 °C in the insulin reduction assay.

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

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This Item
RAB1756AB9328SAB5701494
Thioredoxin human ≥90% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal histidine tagged), essentially salt-free, lyophilized powder, ≥5 U/mg

Sigma-Aldrich

T8690

Thioredoxin human

Human Thioredoxin-1 ELISA for serum, plasma and cell culture supernatants

Sigma-Aldrich

RAB1756

Human Thioredoxin-1 ELISA

Anti-Thioredoxin 1 Antibody serum, Chemicon®

Sigma-Aldrich

AB9328

Anti-Thioredoxin 1 Antibody

Anti-Thioredoxin 1 (Trx1/TXN) antibody produced in rabbit

Sigma-Aldrich

SAB5701494

Anti-Thioredoxin 1 (Trx1/TXN) antibody produced in rabbit

description

≥90% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal histidine tagged), essentially salt-free, lyophilized powder, ≥5 U/mg

description

for serum, plasma and cell culture supernatants

description

serum, Chemicon®

description

-

recombinant

expressed in E. coli (N-terminal histidine tagged)

recombinant

-

recombinant

-

recombinant

-

biological source

human

biological source

-

biological source

rabbit

biological source

rabbit

assay

≥90% (SDS-PAGE)

assay

-

assay

-

assay

-

technique(s)

activity assay: suitable

technique(s)

ELISA: suitable

technique(s)

western blot: suitable

technique(s)

immunohistochemistry: 1:50-1:200, western blot: 1:500-1:2000

suitability

suitable for molecular biology

suitability

-

suitability

-

suitability

-

form

essentially salt-free, lyophilized powder

form

-

form

-

form

liquid

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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