TRUE9

TrueGel3D Hydrogel Kits

Name: TrueGel3D Hydrogel Kits
Brand: SIGMA
Price: 113 USD

SLO-PVA, PEG non cell-degradable crosslinker

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1 KIT

$113.00

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About This Item

NACRES:

NA.71

UNSPSC Code:

12352207

Storage temp.:

−70°C

Shipped in:

dry ice

Key Documents

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shipped in

dry ice

storage temp.

−70°C

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Related Categories

3D Cell Culture Techniques

Cell Culture & Analysis

Hydrogels

Molecular Biology & Functional Genomics

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Show Differences

1 of 4

This Item	TRUE8	TRUE7	TRUE6
Sigma-Aldrich TRUE9 TrueGel3D Hydrogel Kits	Sigma-Aldrich TRUE8 TrueGel3D Hydrogel Kits	Sigma-Aldrich TRUE7 TrueGel3D Hydrogel Kits	Sigma-Aldrich TRUE6 TrueGel3D Hydrogel Kits
storage temp. −70°C	storage temp. −70°C	storage temp. −70°C	storage temp. −70°C
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice

General description

The gel is formed by stable thioether bonds between thiol groups on PEG non cell-degradable crosslinker and thio-reactive groups on SLO-PVA. The polymer, SLO-PVA is functionalized with thiol-reactive groups which react with crosslinker at slower rate than maleimides in FAST-PVA. SLO-PVA does not have sites for cell attachment, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1EA) prior to the crosslinking step.

Application

TRUE9-1KT is used to generate hydrogels of different stiffness (0.15 to 5 KPa shear modulus) to mimic in-vivo conditions and to investigate the effect of matrix stiffness on cell physiology and behavior.SLO-PVA based gels are used to grow cells that do not need attachment to hydrogel for survival but rather use their secreted extracellular matrix. The SLO-PVA based hydrogels are preferred over dextran-based gels for long-term culture of cells producing carbohydrate degrading enzymes. TrueGel3DTM Hydrogel Kits has been used in the generation of 3D gel based tumor models.

Features and Benefits

Slow gelling (takes 6 minutes to 25 minutes, depends on gel stiffness)
Flexibility in modulating gel stiffness
Does not allow cell migration
Does not allow cell recovery and post-culture analyses
Cell inert hydrogel (if not customized with RGD peptide)

Other Notes

TRUE9-1KT contains SLO-PVA solution in phosphate buffer and lyophilized PEG non cell-degradable crosslinker with reconstitution buffer and water.

Disclaimer

Avoid prolong exposure of PEG non cell-degradable crosslinker to air and store SLO-PVA in small portions to avoid frequent freeze-thaw cycles.

pictograms

GHS05

signalword

Warning

hcodes

H290,H315,H319

pcodes

P234 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

wgk

WGK 3

Storage Class

10 - Combustible liquids

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

0001622521

0001615167

0006165167

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Find documentation for the products that you have recently purchased in the Document Library.

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Design of Spherically Structured 3D In vitro Tumor Models-Advances and Prospects

Ferreira LP, et al.

Acta Biomaterialia (2018)

Articles

Hydrogels for 3D Cell Culture

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

TrueGel3D™ Hydrogel for 3D Cell Culture

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

True Gel3D™ Hydrogel Troubleshooting

Troubleshoot TrueGel3D™ hydrogel formation and cell viability issues with RGD-degradable polymer and CD cell-degradable crosslinker.

Protocols

TrueGel3D™ Hydrogel Prep Protocol

TrueGel3D™ is a chemically defined hydrogel for 3D cell growth, facilitating microchannel filling with slow gelation.

Advanced Protocol: How to Customize your TrueGel3D™ Hydrogel

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

Global Trade Item Number

SKU	GTIN
TRUE9-1KT	04061835539321

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