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Rat Vitronectin

Name: Rat Vitronectin
Brand: SIGMA
Price: 529 USD
Availability: InStock

from rat plasma, powder, suitable for cell culture

Synonym(s):

Serum spreading factor

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Size/SKU	Availability	Price
50 μg	Available to ship TODAYfromMILWAUKEE	$529.00

About This Item

CAS Number:

83380-82-9

UNSPSC Code:

12352202

NACRES:

NA.75

MDL number:

MFCD00132575

Biological source:

rat plasma

Form:

lyophilized powder

Technique(s):

cell culture | mammalian: suitable

Mol wt:

75 kDa

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Properties

Product Name

Vitronectin from rat plasma, lyophilized powder, BioReagent, suitable for cell culture

biological source

rat plasma

Quality Segment

200

product line

BioReagent

form

lyophilized powder

mol wt

75 kDa

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

surface coverage

0.1 μg/cm²

solubility

water: soluble 1.00 mL, clear, colorless

UniProt accession no.

Q3KR94

shipped in

dry ice

storage temp.

2-8°C

Gene Information

rat ... Vtn(29169)

Description

General description

Vitronectin, also known as epibolin and S protein, is a 75 kDa protein. It consists of three glycosylation sites. Vitronectin is mainly expressed in plasma.

Application

Vitronectin can be used with cells with integrin receptors that bind vitronectin, including platelets, endothelial cells, melanoma cells, and osteosarcoma. It is recommended as a cell culture substratum at 0.1 μg/cm². Optimal conditions for attachment depend on cell line and application.

Vitronectin has been used as a constituent of serum free media for culturing skeletal muscle and for growth and myelination of motoneurons by Schwann cells. It has also been used as a constituent of enriched co-culture media for culturing rat spinal cord.

Biochem/physiol Actions

Vitronectin inhibits the membrane cytolytic attack complex of the complement system. It interacts with glycosaminoglycans, collagen, plasminogen and the urokinase-receptor. Vitronectin binds to heparin, thrombin and antithrombin III during coagulation and may play a role in the coagulation pathway. It acts a connector between cell adhesion and physiological proteolysis. Vitronectin in association with integrins αvβ3, αvβ5, αvβ1, αIIbβ3, αvβ6 and αvβ8 stimulates cell adhesion, spreading and migration.

Preparation Note

This product is supplied as a cell culture tested lyophilized powder from buffered saline. It is recommended to use polypropylene vessels during handling, due to the strong affinity of vitronectin for hydrophilic surfaces. Reconstitution of this product can be done with 1 mL of water per vial. The stock solution should then be filter-sterilized using a 0.2μm membrane and aliquoted and frozen for long term storage.

Other Notes

Vitronectin is a glycoprotein present in plasma and tissues, and is one of the major cell adhesion proteins, along with fibronectin, in plasma. The N-terminal residues of vitronectin are identical to somatomedin B and are followed by an R-G-D sequence that interacts with a specific cell-surface receptor. The central domain of vitronectin is enriched with hydrophobic residues. Closer to the C-terminus, it has an arginine rich region that allows for heparin binding activity.

Disclaimer

The lyophilized product should be stored desiccated at 2-8°C. Under these conditions it is stable for at least 2 years. Aliquots should be stored at -20°C or -70°C, but repeated freezing and thawing should be avoided.

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This Item	V8379	SRP3186	F6755
Sigma-Aldrich V0132 Vitronectin from rat plasma	Sigma-Aldrich V8379 Vitronectin from human plasma	Sigma-Aldrich SRP3186 Vitronectin human	Sigma-Aldrich F6755 Fibrinogen from rat plasma
description lyophilized powder, BioReagent, suitable for cell culture	description lyophilized powder, BioReagent, suitable for cell culture	description recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture	description 60-80% protein (≥60% of protein is clottable)
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) immunoblotting: suitable, cell culture \| mammalian: suitable
biological source rat plasma	biological source human plasma	biological source human	biological source rat plasma
Quality Level 200	Quality Level 300	Quality Level 200	Quality Level 200
form lyophilized powder	form lyophilized powder	form lyophilized	form powder
mol wt 75 kDa	mol wt B subunit 65 kDa, A subunit 75 kDa	mol wt 52.2 kDa (calculated)	mol wt β-chain 56 kDa, α-chain 63.5 kDa, γ chain 47 kDa (about 4% carbohydrate content), soluble dimer 340 kDa
Gene Information rat ... Vtn(29169)	Gene Information human ... VTN(7448)	Gene Information human ... VTN(7448)	Gene Information rat ... Fga(361969), Fgb(24366), Fgg(24367)

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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