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Anti-Vinculin Antibody

Name: Anti-Vinculin Antibody
Brand: SIGMA
Price: 631 USD
Availability: InStock

Enhanced Validation

rabbit polyclonal

Synonym(s):

Vinculin Antibody - Anti-Vinculin antibody produced in rabbit, Vinculin Antibody Sigma

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Size/SKU	Availability	Price
200 μL	Available to ship TODAYfromMILWAUKEE	$631.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00162905

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IF, WB

Citations:

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Properties

Product Name

Anti-Vinculin antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~116 kDa

species reactivity

rat, human, mouse

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

indirect immunofluorescence: 15-30 μg/mL using acetone-fixed frozen sections of rat or human heart, western blot: 2.5-5 μg/mL using whole extracts of cultured mouse NIH3T3 cells and human hepatoma HepG2 cells

UniProt accession no.

P18206

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)

Description

General description

In muscle, vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), myotendinous junctions (skeletalmuscle), neuromuscular junctions, and the membrane associated dense bodies of smooth muscle. In many cell types undergoing viral transformation, vinculin becomes redistributed to rosettes or podosomes.

Vinculin (VCL) is a ubiquitously expressed cytoskeletal protein, encoded by the gene mapped to human chromosome 10q11.2-qter. VCL is a vital element of adherens type cell junctions. Vinculin structure contains a globular head connected to a tail domain by a short proline-rich sequence, and an intramolecular interaction between the head and tail hides the various ligand-binding sites in the protein.

Immunogen

synthetic peptide corresponding to amino acid residues 691-705 of human vinculin with N-terminal added cysteine, conjugated to KLH. This sequence is also present in metavinculin. The corresponding sequence is identical is mouse, rat, and pig and differs by one amino acid in chicken.

Application

Anti-Vinculin antibody produced in rabbit has been used in immunoprecipitation.

Anti-vinculin antibody produced in rabbit has been used in following studies:

immunofluorescence
immuno-histology
western blotting

Biochem/physiol Actions

Vinculin (VCL) is a cytoskeletal protein that supports cell-to-cell and cell to matrix junctions. It is also essentially involved in normal embryonic development and cardiac function, due to its role in controlling adhesion and motility, as well as cell spreading. In mature focal adhesions (FAs), VCL acts as an important constituent of the “molecular clutch” that induces the transmission of force from cytoplasmic F-actin to membrane-bound integrins. Aberration or mutation of VCL leads to cardiomyopathy, which is associated with hypertension. Experimental studies reveal that, VCL might be used as a biomarker of aging or heart failure. Vinculin associates with FAs and assists in several cellular functions, such as stabilizing and strengthening FAs and promoting their maturation, extracellular matrix (ECM) mechanosensing, regulating actin cytoskeletal dynamics and signaling pathways involved in apoptosis.

Vinculin also binds α-catenin as well as the vasodilator stimulated phosphoprotein (VASP) in focal contact assembly. It transiently binds the actin-related protein-2/3 (ARP2/3) complex without colocalization in focal adhesions.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	V4889	V9131	SAB4200729
Sigma-Aldrich V4139 Anti-Vinculin antibody produced in rabbit	Sigma-Aldrich V4889 Anti-phospho-Vinculin (pTyr⁸²²) antibody produced in rabbit	Sigma-Aldrich V9131 Monoclonal Anti-Vinculin antibody produced in mouse	Sigma-Aldrich SAB4200729 Anti-Vinculin antibody, Mouse monoclonal
description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description clone hVIN-1, ascites fluid	description clone VIN-11-5, purified from hybridoma cell culture
biological source rabbit	biological source rabbit	biological source mouse	biological source mouse
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form ascites fluid	antibody form purified from hybridoma cell culture
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate -
technique(s) western blot: 2.5-5 μg/mL using whole extracts of cultured mouse NIH3T3 cells and human hepatoma HepG2 cells, indirect immunofluorescence: 15-30 μg/mL using acetone-fixed frozen sections of rat or human heart	technique(s) western blot: 1:1,000 using chick embryo fibroblasts (CEFs) transfected with activated Src.	technique(s) western blot: 1:200 using extract of human fibroblasts, indirect immunofluorescence: 1:400 using cultured human fibroblasts, immunohistochemistry (frozen sections): suitable	technique(s) immunoprecipitation (IP): suitable, immunofluorescence: 5-10 μg/mL, immunohistochemistry: suitable, immunoblotting: 0.125-0.25 μg/mL using mouse myoblast C2C12 cell extract
clone polyclonal	clone polyclonal	clone hVIN-1, monoclonal	clone VIN-11-5, monoclonal

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c