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V8012

Anti-V5 antibody, Mouse monoclonal

clone V5-10, purified from hybridoma cell culture

Synonym(s):

V5 Tag Monoclonal Antibody, V5 antibody

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50 μg

Estimated to ship TODAYfromMilliporeSigma Canada Ltd.

$589.00

About This Item

NACRES:
NA.56
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
V5-10, monoclonal
Application:
ICC, WB
Citations:
144

$589.00

Estimated to ship TODAYDetails

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biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

V5-10, monoclonal

concentration

~1 mg/mL

technique(s)

immunocytochemistry: 1-2 μg/mL, western blot: 0.5-1 μg/mL

shipped in

dry ice

storage temp.

−20°C

General description

Monoclonal Anti-V5 (mouse IgG1 isotype) is derived from the V5-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide conjugated to KLH.
The mouse monoclonal Anti-V5 antibody reacts specifically with the V5-tagged recombinant fusion proteins expressed in transfected mammalian cells or produced by in vitro translation.
The mouse mooclonal Anti-V5 antibody reacts specifically with the V5-taged recombinant fusion proteins expressed in transfected mammalian cells or produced by in vitro translation.

Biochem/physiol Actions

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-V5 antibody, Mouse monoclonal clone V5-10, purified from hybridoma cell culture

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Anti-V5-Cy3™ antibody, Mouse monoclonal purified immunoglobulin, ~1 mg/mL, clone V5-10

Sigma-Aldrich

V4014

Anti-V5-Cy3™ antibody, Mouse monoclonal

Monoclonal Anti-V5-Peroxidase antibody produced in mouse clone V5-10, purified immunoglobulin, lyophilized powder

Sigma-Aldrich

V2260

Monoclonal Anti-V5-Peroxidase antibody produced in mouse

Anti-Vimentin antibody, Mouse monoclonal clone V9, purified from hybridoma cell culture

Sigma-Aldrich

V6389

Anti-Vimentin antibody, Mouse monoclonal

conjugate

unconjugated

conjugate

CY3 conjugate

conjugate

peroxidase conjugate

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

clone

V5-10, monoclonal

clone

V5-10, monoclonal

clone

V5-10, monoclonal

clone

V9, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

technique(s)

immunocytochemistry: 1-2 μg/mL, western blot: 0.5-1 μg/mL

technique(s)

immunocytochemistry: 2.0-4.0 μg/mL

technique(s)

western blot: 1:4,000-1:8,000

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 25-50 μg/mL using human tissue, microarray: suitable, western blot: 0.2-0.5 μg/mL using human fibroblasts HS-68

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000561216
0000561216
0000463835
0000463835
0000488170

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