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V8137

Anti-V5 antibody produced in rabbit

IgG fraction of antiserum

Anti-V5 antibody produced in rabbit
1 of 1 reviewers received a sample product or took part in a promotion

Synonym(s):

Rabbit Anti-V5

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Size/SKUAvailabilityPrice
0.2 mg
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₹55,666.50

About This Item

UNSPSC Code:
12352203
NACRES:
NA.56
MDL number:
MFCD03456076
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, IP, WB
Citations:
121

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biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

technique(s)

immunocytochemistry: 5 μg/mL using V5 tagged fusion protein in methanol-acetone fixed transiently transfected 293T cells, immunoprecipitation (IP): 5 μg/mL using V5 tagged fusion protein from extracts of transfected cells, western blot: 2.5 μg/mL using V5 tagged fusion protein expressed in whole extracts of transfected cells

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

V5 is an amino coterminal protein consisting of a C-terminus cysteine-rich region that resembles a cysteine-rich metal binding domain. It is encoded by the "P" gene of the paramyxovirus SV5.
The rabbit Anti-V5 synthetic peptide antibody reacts with V5 tagged recombinant fusion proteins expressed in transfected mammalian cells and from in vitro translation.

Application

Anti-V5 antibody produced in rabbit has been used in:
  • immunocytochemistry
  • immunoprecipitation
  • western blot

Biochem/physiol Actions

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Physical form

Solution in 0.01M phosphate buffered saline, pH 7.4 containing 15 mM sodium azide.

Preparation Note

Store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Sigma-Aldrich

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Sigma-Aldrich

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Sigma-Aldrich

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Sigma-Aldrich

H6908

Anti-HA antibody produced in rabbit

description

IgG fraction of antiserum

description

affinity isolated antibody, buffered aqueous solution

description

IgG fraction of antiserum, buffered aqueous solution

description

affinity isolated antibody, buffered aqueous solution

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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Storage Class

10 - Combustible liquids

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flash_point_f

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flash_point_c

Not applicable

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1 Review This action will navigate to reviews.

Questions

  1. What is the concentration of this antibody? I understand each vial contains 0.2mg in a phosphate buffer, but what is the volume or concentration?

    1 answer

    1. The IgG concentration of this antibody is lot-specific and may range from 4.0–5.0 mg/mL. The 0.2 mg vial volume may range from 40–50 µL. Please see the link below to review additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/246/723/v8137dat.pdf

      Please see the following link to review a sample or lot-specific Certificate of Analysis:
      https://www.sigmaaldrich.com/product/sigma/v8137#product-documentation

      Helpful?

1 Review This action will navigate to reviews.
1 of 1 reviewers received a sample product or took part in a promotion
1 out of 1 (100%) reviewers recommend this product

Reviews

Active Filters

  1. Salt Lake City, Utah, USA
    • Reviews 2
    • Votes 2
    3 out of 5 stars.

    Research Scientist

    It is a good antibody for Western Blot for both specimen of human and mouse. But it often shows signals medium intensity, not strong enough.

    Helpful?

    1. Response from Merck KGaA:

      Thank you for taking the time to leave a review! We would like to learn more about your experience to see if there is anything we can do to help troubleshoot this issue. When you have a moment, please contact us by visiting https://www.sigmaaldrich.com/support/customer-support and submit a Report Product Issue ticket. We look forward to hearing from you soon!