W0138
Western Blocker™ Solution
for HRP detection systems
Synonym(s):
Western blot blocking solution, blocking buffer
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| Size/SKU | Availability | Price |
|---|---|---|
400 mL | Available to ship TODAYfromMILWAUKEE | $134.00 |
About This Item
NACRES:
NA.25
UNSPSC Code:
41105329
eCl@ss:
32110600
$134.00
Available to ship TODAYDetails
General description
Western Blocker Solution is used to block both PVDF and nitrocellulose membranes after proteins have been transferred onto them through dot blotting or Western blotting; blocking is performed to reduce or eliminate nonspecific binding of primary or secondary antibodies.
Application
Western Blocker Solution has been optimized for detection systems using horseradish peroxidase (HRP) conjugates; it is also compatible with alkaline phosphatase based systems.
Western Blocker Solution has been used for blocking of western blot membranes. It has also been used for dilution of antibodies in western blot.
Western Blocker Solution has been used for blocking of western blot membranes. It has also been used for dilution of antibodies in western blot.
Features and Benefits
- Does not inhibit weak HRP signals, as many milk-based blockers do.
- Can be used both as a blocking component and as a probing component.
- Supplied ready-to-use; no preparative step is required.
Legal Information
Western Blocker is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
|---|---|---|---|
| description for HRP detection systems | description Fluorescent Detection for Western blotting, dot/slot blotting, mass spectrometry and fluorescence assays | description size (square), size 11.4 cm × 11.4 cm × 3.2 cm, pk of 5 | description clear, size 7.3 cm × 5.1 cm × 3.2 cm, pk of 5 |
| technique(s) immunodetection: suitable | technique(s) western blot: suitable, immunodetection: suitable | technique(s) - | technique(s) - |
| storage temp. 2-8°C | storage temp. 15-25°C | storage temp. - | storage temp. - |
| shelf life ≥2 yr | shelf life - | shelf life - | shelf life - |
| Quality Level 200 | Quality Level 300 | Quality Level - | Quality Level - |
Storage Class
12 - Non Combustible Liquids
WGK
WGK 2
Hazard Classifications
ED ENV 1
Signal Word
Danger
Hazard Codes
Precautionary Statements
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