Key Documents

XTT sodium salt

Name: XTT sodium salt
Brand: SIGMA
Price: 352 USD
Availability: InStock

powder, BioReagent, suitable for cell culture

Synonym(s):

2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt, 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt

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Size/SKU	Availability	Price
100 mg	Check Cart for Availability	$352.00
500 mg	Check Cart for Availability	$1,190.00

About This Item

Empirical Formula (Hill Notation):

C₂₂H₁₆N₇NaO₁₃S₂

CAS Number:

111072-31-2

Molecular Weight:

673.52

NACRES:

NA.75

PubChem Substance ID:

24902150

UNSPSC Code:

12352207

MDL number:

MFCD00083517

$352.00

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Properties

product line

BioReagent

Quality Segment

200

assay

≥90%

form

powder

storage condition

dry at room temperature

technique(s)

cell culture | mammalian: suitable, protein quantification: suitable

application(s)

detection

detection method

colorimetric

storage temp.

2-8°C

SMILES string

[Na+].COc1cc(c(cc1-n2nc(n[n+]2-c3cc(c(cc3OC)[N+]([O-])=O)S([O-])(=O)=O)C(=O)Nc4ccccc4)S([O-])(=O)=O)[N+]([O-])=O

InChI

1S/C22H17N7O13S2.Na/c1-41-17-8-15(28(31)32)19(43(35,36)37)10-13(17)26-24-21(22(30)23-12-6-4-3-5-7-12)25-27(26)14-11-20(44(38,39)40)16(29(33)34)9-18(14)42-2;/h3-11H,1-2H3,(H2-,23,30,35,36,37,38,39,40);/q;+1/p-1

InChI key

JACYMBNQPPWQML-UHFFFAOYSA-M

Description

General description

XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is a tetrazolium salt, which forms water-soluble formazan on bioreduction.

Application

XTT sodium salt has been used to assess cell viability.

XTT has been used in conjunction with phenazine methosulfate (PMS) to screen human cancer cell lines. The tetrazolium dye has also been used to study fungal cell damage, in testing antimicrobial susceptibility of staphylococci, and in Candida biofilm research. Limitations to the XTT assay have been reported.

Biochem/physiol Actions

XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is acted upon by dehydrogenases present in metabolically active cells. This produces water soluble formazan, which is a highly colored product. Thus, it offers an advantage in colorimetric proliferation assays, as opposed to MTT, as the product is already soluble and no formazan crystals are formed.

In living cells, XTT is metabolically reduced to produce a colorimetric, water-soluble formazan product.

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Show Differences

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This Item	E6511	11585029001	N6639
Sigma-Aldrich X4626 XTT sodium salt	Sigma-Aldrich E6511 Ethylenediaminetetraacetic acid tetrasodium salt dihydrate	Roche 11585029001 NBT	Sigma-Aldrich N6639 Nitrotetrazolium Blue chloride
description powder, BioReagent, suitable for cell culture	description BioReagent, suitable for cell culture, 98.5-102.0%	description crystals, pkg of 5 g	description powder, electrophoresis grade
technique(s) protein quantification: suitable, cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) -	technique(s) electrophoresis: suitable
detection method colorimetric	detection method -	detection method -	detection method -
storage condition dry at room temperature	storage condition -	storage condition -	storage condition -
form powder	form powder	form crystals	form powder
application(s) detection	application(s) -	application(s) -	application(s) -
storage temp. 2-8°C	storage temp. room temp	storage temp. 2-8°C	storage temp. 2-8°C

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Pictograms

GHS02

Signal Word

Danger

Hazard Codes

H242

Precautionary Statements

P210 - P234 - P235 - P240 - P370 + P378 - P403

Hazard Classifications

Self-react. C

supp_hazards

EUH044

Storage Class

4.1A - Other explosive hazardous materials

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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