Y0750
Yeast Synthetic Drop-out Medium Supplements
without leucine and tryptophan
Synonym(s):
Synthetic Yeast Nutritional Supplements
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| Size/SKU | Availability | Price |
|---|---|---|
20 g | Check Cart for Availability | $425.00 |
About This Item
$425.00
List Price$500.00Save 15%Check Cart for Availability
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Application
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Preparation Note
1.54 g will supplement 1 liter of medium.
Other Notes
Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.
The supplements contain all of the following components except as indicated:
Amino acids: All standard amino acids except for leucine and tryptophan. All the amino acids are present at a concentration of 76 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L), uracil (76 mg/L).
Amino acids: All standard amino acids except for leucine and tryptophan. All the amino acids are present at a concentration of 76 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L), uracil (76 mg/L).
1 of 1
This Item | |||
|---|---|---|---|
| description without leucine and tryptophan | description without uracil | description without histidine, leucine, and tryptophan | description without uracil, leucine, and tryptophan |
| form powder | form powder | form powder | form powder |
| application(s) food and beverages | application(s) food and beverages | application(s) microbiology | application(s) microbiology |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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