DTT-RO

DTT

crystalline powder, =97% (Ellman′s reagent), M_r 154.3

• Synonym(s): DL-Dithiothreitol, 1,4-dithiothreitol, cleland′s reagent, dithiothreitol, 1,4-, threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT
• Linear Formula: HSCH₂CH(OH)CH(OH)CH₂SH
• CAS Number: 3483-12-3
• Molecular Weight: 154.25
• Beilstein: 1719757
• Número MDL: MFCD00004877
• ID de substância PubChem: 329798930

Properties

• descrição: 1,4-Dithiothreitol
• teor: =97% (Ellman′s reagent)
• forma: crystalline powder
• peso molecular: M_r 154.3
• reaction suitability: reagent type: reductant
• embalagem: pkg of 10 g (10708984001); pkg of 2 g (10197777001); pkg of 25 g (11583786001)
• manufacturer/tradename: Roche
• pf: 41-44 °C (lit.)
• temperatura de armazenamento: 2-8°C
• SMILES string: O[C@H](CS)[C@H](O)CS
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
• InChI key: VHJLVAABSRFDPM-QWWZWVQMSA-N

Description

Descrição geral

1,4-dithiothreitol is commonly known as Cleland′s reagent. It confers protection to thiol groups and reduces disulfide bonds in peptides and proteins. It reduces disulfide bonds to sulfhydryl group and is commonly used in receptor studies, to determine the functional importance of disulfide bonds during receptor occupancy and functionality. It also maintains monothiols completely in the reduced state.

Aplicação

1,4-dithiothreitol is used in:

• Isolation, purification and characterization of proteins and enzymes
• Measurement of enzyme activities (reactivation of enzymes)
• Determination of disulfide groups in proteins and enzymes
• DNA extraction prior to amplification

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Especificações

Oxidized form: <2.5% (absorbance at 283nm)

Sequência

Enantiomers
Our DTT-preparation is optically inactive, i.e. it is the D,L-DTT.

Nota de preparo

Storage conditions (working solution): A solution of DTT in Hepes buffer, pH 7.75 is stable for one week at 2 to 8 °C if the container is tightly sealed and the solution is protected from atmospheric oxygen by argon or nitrogen.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Pictogramas: GHS05,GHS07
• Palavra indicadora: Danger
• Frases de perigo: H302 - H315 - H318 - H412
• Declarações de precaução: P264 - P270 - P273 - P280 - P305 + P351 + P338 + P310 - P501
• Classificações de perigo: Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2
• Código de classe de armazenamento: 11 - Combustible Solids
• WGK: WGK 2