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76216

Papain from Carica papaya

solution, light brown, ≥10 U/mg protein (~25 mg/ml)

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Para você/SKUDisponibilidadePreço
50 mg

Disponível para envio HOJEdeCajamar

R$ 565,00
250 mg

Disponível para envio HOJEdeCajamar

R$ 1.794,00
1 g

Disponível para envio HOJEdeCajamar

R$ 6.478,00

Sobre este item

Número CAS:
UNSPSC Code:
12352204
eCl@ss:
42030311
EC Number:
232-627-2
NACRES:
NA.54
MDL number:
Número CE:
Specific activity:
≥10 U/mg protein (~25 mg/ml)
Biological source:
papaya

R$ 565,00


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biological source

papaya

Quality Segment

form

solution

specific activity

≥10 U/mg protein (~25 mg/ml)

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

color

light brown

storage temp.

2-8°C

General description

Papain is a proteolytic enzyme and is naturally occurring in papaya (Carica papaya L.). It is obtained from the latex of cut unripe papaya. Papain belongs to the cysteine proteinase family. It is a globular, single-chain protein composed of four disulfide bridges and catalytically essential residues. Papain consists of an all α-domain and anti-parallel β-sheet domain.

Application

Papain is used as a meat tenderizer. It′s also used to break up tissue in the preparation of primary cell cultures.
Papain from Carica papaya has been used:

  • in the enzymatic dissociation of rat cortices to extract astrocytes for cell culture
  • to study its effects on Alpaca seminal plasma viscosity and sperm function
  • to study its effects on the yield and cryo-survival of Rhinoceros spermatozoa[1]

Biochem/physiol Actions

Papain is a cysteine protease that cleaves peptide bonds and is known to cleave the Fc portion of antibodies.
Papain plays an important role in many biological processes in many living organisms. It exhibits proteolytic activity against proteins, amino-acid esters and amide links, and short-chain peptides. Papain preferentially cleaves the peptide bonds of basic amino acids such as arginine, phenylalanine, and lysine. It has a wide range of applications in drug discovery, pharmaceutical, food, baking, and dairy industries.

Other Notes

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol N-benzoyl-L-arginine ethyl ester (BAEE, Cat. No. 12880) per minute at pH 6.2 and 25°C

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Este Item
P4762P3125P3375
specific activity

≥10 U/mg protein (~25 mg/ml)

specific activity

≥10 units/mg protein

specific activity

≥16 units/mg protein

specific activity

1.5-10 units/mg solid

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

technique(s)

-

technique(s)

-

biological source

papaya

biological source

papaya (latex)

biological source

-

biological source

-

form

solution

form

lyophilized powder

form

buffered aqueous suspension

form

crude powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

100

Quality Level

300

Quality Level

200

Quality Level

200


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pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Classe de armazenamento

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves



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Questions

  1. Which solution is used in Papain from Carica papaya solution? DMSO? water?

    1 answer
    1. This product is an aqueous solution containing 35 - 55% glycerol.

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