Flow injection spectrophotometric determination of L-ascorbic acid in biological matters.

A method for spectrophotometric determination of L-ascorbic acid (vitamin C) in tissues by flow injection analysis is reported. The procedure is based on the oxidation of analyse with iron(III) and 2,2'-dipyridyl. Concentrations of vitamin C in the range of 0.5-20 ppm have been determined with a relative standard deviation 1.2% (n=15). The injection rate was 40 samples per h. The method was applied to the determination of L-ascorbic acid in rat's tissues (blood serum, brain, and liver) and compared favourably with an independent reference method based on spectrophotometry.