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C9268
Carboxypeptidase A from bovine pancreas
(Type II-PMSF treated), ≥50 units/mg protein, ready-to-use solution
Synonyme(s) :
Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase
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| Taille/Référence | Disponibilité | Prix |
|---|---|---|
500 units | Vérifier la disponibilité des articles du panier | 119,00 € |
2500 units | Vérifier la disponibilité des articles du panier | 401,00 € |
5000 units | Vérifier la disponibilité des articles du panier | 435,00 € |
A propos de cet article
Numéro CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
Numéro CE :
MDL number:
Specific activity:
≥50 units/mg protein
Service technique
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Proteomics Grade
Quality Segment
form
ready-to-use solution
quality
(Type II-PMSF treated)
specific activity
≥50 units/mg protein
mol wt
~35 kDa
purified by
2× crystallization
impurities
≤0.05 BTEE units/mg protein chymotrypsin, ≤10 BAEE units/mg protein trypsin
storage temp.
2-8°C
Application
The enzyme from Sigma has been used as a comparison to study the specificity of Metarhizium anisopliae carboxypeptidase A (MeCPA). MeCPA had been genetically engineered to facilitate the removal of polyhistidine tags from the C-termini of recombinant proteins. It has also been used to de-tyrosinate α-tubulin, in vitro, in order to induce high affinity to ethyl-N-phenylcarbamate (EPC) sepharose.
Carboxypeptidase A from bovine pancreas has been used in a study to investigate the expression of a soluble and activatable form of bovine procarboxypeptidase A in Escherichia coli. Carboxypeptidase A from bovine pancreas has also been used in a study to investigate the isolation and partial characterization of precursor forms of ostrich carboxypeptidase.
Biochem/physiol Actions
Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by beta-phenylpropionate and indole acetate.
Preparation Note
Treated with phenylmethylsulfonyl fluoride to eliminate trypsin and chymotrypsin activity. Dialyzed and recrystallized: aqueous suspension with toluene added.
Analysis Note
Protein determined by E1%/278
Other Notes
One unit will hydrolyze 1.0 μmole of hippuryl-L-phenylalanine per min at pH 7.5 at 25 °C.
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Cet article | |||
|---|---|---|---|
| description (Type II-PMSF treated), ≥50 units/mg protein, ready-to-use solution | description (Type II-PMSF treated), ≥20 units/mg protein | description ammonium sulfate suspension, ≥6 units/mL packed gel, 25 °C, enzyme from bovine pancreas | description lyophilized powder |
| specific activity ≥50 units/mg protein | specific activity ≥20 units/mg protein | specific activity ≥6 units/mL packed gel, 25 °C | specific activity ≥125 units/mg protein |
| grade Proteomics Grade | grade - | grade - | grade Proteomics Grade |
| form ready-to-use solution | form aqueous suspension | form ammonium sulfate suspension | form lyophilized powder |
| mol wt ~35 kDa | mol wt ~35 kDa | mol wt ~35,250 | mol wt 34,000 Da± 600 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Mot-clé
Danger
Codes de danger
Hazard Classifications
Resp. Sens. 1
Classe de stockage
10 - Combustible liquids
Que se passe-t-il ?
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
EPI (équipement de protection individuelle)
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Listes réglementaires
Les listes réglementaires sont principalement fournies pour les produits chimiques. Seules des informations limitées peuvent être fournies ici pour les produits non chimiques. L'absence d'indication signifie qu'aucun des composants n'est répertorié. Il incombe à l'utilisateur de s'assurer de l'utilisation sûre et légale du produit.
108-88-3
CAS No.
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