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Merck

1.02129

Chromolith® RP-18 endcapped (monolithic) HPLC Columns

L × I.D. 100 mm x 4.6 mm HPLC Column

Sinonimo/i:

Chromolith® RP-18 endcapped

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Informazioni su questo articolo

Codice UNSPSC:
41115709
NACRES:
SB.52

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Nome del prodotto

Colonna per HPLC Chromolith® Performance RP-18 endcapped 100-4,6, L × I.D. 100 mm x 4.6 mm HPLC column

Materiali

PEEK column

Livello qualitativo

agenzia

suitable for USP L1

Nome Commerciale

Chromolith®

Caratteristiche

endcapped

Confezionamento

1 ea of

Grado di funzionalizzazione

18% Carbon loading

Parametri

200 bar pressure
45 °C max. temp.

tecniche

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

Lungh. × diam. int. colonna

10 cm × 4.6 mm

Matrice

monolithic silica

Gruppo funzionale matrice

C18 bonding phase

Dimensione pori

13 nm mesoporosity
2 μm macroporosity

Intervallo di pH d’impiego

2-7.5

Tecnica di separazione

reversed phase

Temperatura di conservazione

no temp limit

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Questo articolo
1.520061.520011.51450
matrix active group

C18 bonding phase

matrix active group

C18 bonding phase

matrix active group

C18 bonding phase

matrix active group

C18 bonding phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

pore size

13 nm mesoporosity, 2 μm macroporosity

pore size

1.5 μm macroporosity, 13 nm mesoporosity

pore size

13 nm mesoporosity, 2 μm macroporosity

pore size

13 nm mesoporosity, 2 μm macroporosity

technique(s)

HPLC: suitable, mass spectrometry (MS): suitable (High), LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable, mass spectrometry (MS): suitable (High)

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

matrix

monolithic silica

matrix

monolithic silica

matrix

monolithic silica

matrix

monolithic silica

Descrizione generale

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® Performance RP-18 endcapped HPLC columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å) and the surface modification is octadecylsilane with full end-capping.

Chromolith® Performance RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column.

Chromolith®Performance RP-18 endcapped columns are perfectly suitable for LC-MS applications. The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for separations of complex samples on any HPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:

  • Rapid separations at very low column back-pressure
  • High matrix tolerance, allowing time and cost savings with simplified workflows
  • Cost savings due to extended column lifetimes
  • Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are: Chromolith® RP-18 endcapped 10-4.6 guard cartridges (3 pieces) [1.51452] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032] Or Chromolith® RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.51451] with the Chromolith® Guard Cartridge Holder 10 mm x 4.6 mm [1.52033] The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467] Need more efficiency? Chromolith®HighResolution RP-18 endcapped 100-4.6 HPLC columns [1.52022] provide a column efficiency exceeding 140,000 plates/meter. Learn more: Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments. Supelco HPLC Products: The Best Choice for Any LC Instrument

Applicazioni


  • L'inquinamento salino riduce la torbidità, la materia organica disciolta e i cianobatteri in comunità sperimentali di pozze vernali. Lo studio discute le applicazioni ambientali del cloruro di calcio, concentrandosi sul suo uso nella gestione degli ecosistemi colpiti dall'inquinamento salino, dimostrando il suo ruolo nella bonifica e nella gestione ambientale (Vigil and Schuler, 2024).



Risultati analitici

Aspetto: conforme
Contenuto in carbonio: 17,0 - 18,6 %
Pressione: ≤ 30 bar
Test di stabilità: conforme
Prestazione (antracene)
Piatti teorici (N/m) (antracene): ≥ 80.000
Fattore di ritenzione (antracene): 3,9 - 4,6
Scodatura (USP): 0,90 - 1,60%
Selettività
Fattore di separazione (antracene/progesterone): 1,95- 2,35

Note legali

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

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Codice della classe di stoccaggio

10-13 - German Storage Class 10 to 13


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

LC-UV-MS workflow details teriparatide peptide mapping, including enzymatic digestion, separation conditions, and QTOF mass spectrometer identification.

Chromolith® RP-18e columns optimize Oligo Standard 6 separation with varied flow rates and ion-pairing reagent evaluation.

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

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Domande

1–3 di 3 domande  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 risposta
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Utile?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 risposta
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Utile?

  3. What is the particle size of Chromolith 18 endcap column

    1 risposta
    1. This product is not comprised of distinct particles; rather it is a a monolithic silica column. This silica monolith contains both mesopores (~13 nm) and macropores (~2 um) Please see the following for more information:
      https://www.sigmaaldrich.com/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/chromolith-hplc-and-uhplc-columns

      Utile?

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