48-660MAG

MILLIPLEX MAPK/SAPK Signaling 10-Plex Kit - Cell Signaling Multiplex Assay

Name: MILLIPLEX MAPK/SAPK Signaling 10-Plex Kit - Cell Signaling Multiplex Assay
Brand: MM
Price: 3610 EUR
Availability: InStock

Sinonimo/i:

Cell Signaling Kit, MAPK/SAPK 10-Plex Kit

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96 WELLS

3.610,00 €

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Informazioni su questo articolo

Codice UNSPSC:

12161503

eCl@ss:

32161000

NACRES:

NA.47

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Reattività contro le specie

human

Livello qualitativo

200

Produttore/marchio commerciale

Milliplex^®

tecniche

multiplexing: suitable

assay range

inter-assay cv: 4-13%
intra-assay cv: 3-10%

Metodo di rivelazione

fluorometric (Luminex^® xMAP^® technology)

Temperatura di conservazione

2-8°C

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Questo articolo	48-610MAG	48-625MAG	48-612MAG
Millipore 48-660MAG MILLIPLEX MAPK/SAPK Signaling 10-Plex Kit - Cell Signaling Multiplex Assay	Millipore 48-610MAG MILLIPLEX MAP STAT Cell Signaling Magnetic Bead 5-Plex Kit - Cell Signaling Multiplex Assay	Millipore 48-625MAG Saggio multiplex MILLIPLEX^® Phospo/Total mTOR 2-Plex per studi di segnalazione cellulare	Millipore 48-612MAG Kit MILLIPLEX^® a 11 analiti con sferette magnetiche per le fosfoproteine totali della via Akt/mTOR - Saggio multiplo sulla trasduzione intracellulare del segnale
species reactivity human	species reactivity human	species reactivity human, rat, mouse	species reactivity human
assay range inter-assay cv: 4-13% intra-assay cv: 3-10%	assay range -	assay range -	assay range -
manufacturer/tradename Milliplex^®	manufacturer/tradename Milliplex^®	manufacturer/tradename Milliplex^®	manufacturer/tradename Milliplex^®
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
technique(s) multiplexing: suitable	technique(s) multiplexing: suitable	technique(s) multiplexing: suitable	technique(s) multiplexing: suitable
detection method fluorometric (Luminex xMAP)	detection method fluorometric (Luminex xMAP)	detection method fluorometric (Luminex^® xMAP^®)	detection method fluorometric (Luminex^® xMAP^®)

Descrizione generale

Cells respond to their environment in various ways through intracellular signaling. Signaling through Receptor Tyrosine kinases (RTKs) often promotes increased metabolism and cell growth. Activation of ERK/MAP kinase is one of the key serine/threonine kinases activated via RTK signaling, promoting increased activity of p70 S6 kinase, MSK1, STATs, CREB, and many other signaling intermediates. Other signaling pathways induced via stress (ex. heat shock/arsenite treatment) or death receptors (ex. TNFα) promote p38, JNK, and p53 signaling pathways.

Applicazioni

An overnight (4°C) incubation is recommended for best results.
This assay requires 25 μL diluted cell lysate.
This kit must be run using Assay Buffer 2 (provided).
1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg cell lysate/well).

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples.

Research Category
Signaling

Azioni biochim/fisiol

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Note legali

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Pittogrammi

GHS05,GHS07,GHS09

Avvertenze

Danger

Indicazioni di pericolo

H302,H315,H318,H410

Consigli di prudenza

P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338

Classi di pericolo

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

Codice della classe di stoccaggio

10 - Combustible liquids

Certificati d'analisi (COA)

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Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 246-247, 110461-110461 (2020-06-05)

In arid conditions, the African Clawed frog Xenopus laevis enters a state of estivation dormancy as an adaptive survival strategy. Under estivation, X. laevis experience severe dehydration stress as 25-35% of total body water is lost. Dehydration in X. laevis

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