506119

PhosphoDetect Anti-p38 MAP Kinase (pThr¹⁸⁰, pTyr¹⁸²) Rabbit pAb

liquid, Calbiochem^®

• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Stato: liquid
• contiene: ≤0.1% sodium azide as preservative
• Reattività contro le specie: rat, mouse, canine, human
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; avoid repeated freeze/thaw cycles
• Isotipo: IgG
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: phosphorylation (pThr180/pTyr182)
• Informazioni sul gene: human ... MAPK14(1432)

Descrizione

Descrizione generale

Note: 1 T = 1 test. Sufficient for 10 Western miniblots.

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~p38 MAPK phosphorylated at Thr¹⁸⁰ and Tyr¹⁸².

Recognizes p38α, β, and γ/ERK6 phosphorylated at Thr¹⁸⁰ and Tyr¹⁸² in uv-treated HEK293 cells and sorbitol-treated PC12 cells.

This PhosphoDetect Anti-p38 MAP Kinase Rabbit pAb is validated for use in WB, ICC for the detection of p38 MAP Kinase .

Immunogeno

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸⁰ and Tyr¹⁸² phosphorylation sites of human p38 MAP Kinase

Applicazioni

Immunoblotting (1:1000)

Immunocytochemistry (see comments)

Stato fisico

In Dulbecco′s PBS (without Mg²⁺ and Ca²⁺), 1 mg/ml BSA, 50% glycerol, pH 7.3.

Nota sulla preparazione

Following initial thaw, aliquot and freeze (-20°C).

Risultati analitici

Positive Control
UV irradiated HEK293 cells or sorbitol-treated PC12 cells

Altre note

Does not appreciably recognize the corresponding phosphorylated forms of either p42/44 MAPK or SAPK/JNK MAPK. The immunogen sequence is 100% conserved in p38α, β, and γ. The immunogen sequence is also 100% conserved in mouse, moneky, and carp p38, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. When diluted 1:1000, there is sufficient antibody for 10 immunoblots at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.

Marinissen, M.J., et al. 2001. Genes Dev.15, 535.
Nakagami, H., et al. 2001. Diabetes50, 1472.
Wilsbacher, J.L., et al. 1999. J. Biol. Chem.274, 16988.
Raingeaud, J., et al. 1995. J. Biol. Chem.270, 7420.
Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA92, 10531.
Han, J., et al. 1994. Science265, 808.
Lee, J.C., et al. 1994. Nature372, 739.
Rouse, J., et al. 1994. Cell78, 1027.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Toxicity: Standard Handling (A)

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable