AB10181

Anti-Pumilio-2 (PUM2) Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: pumilio homolog 2 (Drosophila), pumilio homolog 2, pumilio (Drosphila) homolog 2
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Coniugato: unconjugated
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: rhesus macaque, mouse, human
• Reattività contro le specie (prevista in base all’omologia): chimpanzee (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology), canine (based on 100% sequence homology)
• tecniche: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• N° accesso NCBI: NP_056132
• N° accesso UniProt: Q8TB72
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... PUM2(23369)

Descrizione

Descrizione generale

PUM2 is a member of the Puf family of RNA-binding proteins, which are believed to play a role in the regulation of posttranscriptional gene expression. Members of the Puf family of RNA binding proteins function as repressors of translation when bound to the 3-untranslated region of RNA transcripts.

~120 kDa observed.
Non specific band may be observed at 48 kDa

Immunogeno

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of human Pumilio-2.

Applicazioni

Detect Pumilio-2 (PUM2) using this Anti-Pumilio-2 (PUM2) Antibody validated for use in IC, IH & WB.

Immunohistochemistry Analysis: A 1:400 dilution from a previous lot detected Pumilio-2 in human testis and teratoma tissue.

Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Pumilio-2 in human and mouse embryonic stem cells.

Research Category
Stem Cell Research

Research Sub Category
RNA Metabolism & Binding Proteins

Azioni biochim/fisiol

This antibody recognizes Pumilio-2 near the N-terminus.

Stato fisico

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Nota sulla preparazione

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
Mouse embryonic stem cell lysate

Evaluated by Western Blot on mouse embryonic stem cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Pumilio-2 (PUM2) in 10 µg of mouse embryonic stem cell lysate.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable