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Merck

AB5441

Anti-Tropomyosin Antibody

Chemicon®, from sheep

Sinonimo/i:

Anti-C15orf13, Anti-CMD1Y, Anti-CMH3, Anti-HEL-S-265, Anti-HTM-alpha, Anti-LVNC9, Anti-TMSA

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100 μL

398,00 €

398,00 €


Spedizione prevista il28 dicembre 2026Dettagli

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Informazioni su questo articolo

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

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Nome del prodotto

Anti-Tropomyosin Antibody, Chemicon®, from sheep

biological source

sheep

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

Gene Information

rat ... Tpm3(117557)

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T2780T9283SAB1400284
antibody form

affinity purified immunoglobulin

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified immunoglobulin

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

biological source

sheep

biological source

mouse

biological source

mouse

biological source

mouse

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

technique(s)

immunocytochemistry: suitable, western blot: suitable, immunohistochemistry: suitable

technique(s)

immunoprecipitation (IP): suitable, indirect ELISA: suitable, indirect immunofluorescence: 1:400 using chicken fibroblasts, microarray: suitable, western blot: suitable using human tissue and chicken gizzard extracts

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 using sections of human tongue, immunohistochemistry (frozen sections): suitable using human and animal tissue sections, immunoprecipitation (IP): suitable, microarray: suitable, western blot: suitable using native preparations

technique(s)

immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, indirect ELISA: suitable, western blot: 1-5 μg/mL

Gene Information

rat ... Tpm3(117557)

Gene Information

bovine ... TPM1(281544)
chicken ... TPM1(396366)
human ... TPM1(7168)
mouse ... Tpm1(22003)
rat ... Tpm1(24851)

Gene Information

chicken ... TPM1(396366)
human ... TPM1(7168)
rat ... Tpm1(24851)

Gene Information

human ... TTN(7273)

Application

Research Category
Metabolism
Research Sub Category
Muscle Physiology
This Anti-Tropomyosin Antibody is validated for use in WB, IC, IH for the detection of Tropomyosin.
Western Blot: 1:200-1:1,000. The antibody reacts with the ~29 kDa Tropomyosin protein on mouse brain cytosol. An additional band at ~52-55 kDa may be seen depending on sample and antibody concentration used. It is thought that this band may be a dimer.
Immunocytochemistry: 1:100-1:500
Immunohistochemistry: 1:100-1:500


Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes Tropomyosin 1, 2, 3, 5a, 5b, 6.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Synthetic peptide corresponding to the 9d exon from the alpha gene of tropomyosin.

Physical form

Affinity purified immunoglobulin. Liquid in PBS.

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Classe di stoccaggio

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

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Eui-Jung Park et al.
American journal of physiology. Renal physiology, 304(7), F958-F971 (2013-01-11)
It has been reported that several proteins [heat shock protein 70 (Hsp70 and Hsc70), annexin II, and tropomyosin 5b] interact with the Ser(256) residue on the COOH terminus of aquaporin-2 (AQP2), where vasopressin-induced phosphorylation occurs for mediating AQP2 trafficking. However
Functional effects of mutations in the tropomyosin-binding sites of tropomodulin1 and tropomodulin3.
Lewis, RA; Yamashiro, S; Gokhin, DS; Fowler, VM
Cytoskeleton (Hoboken, N.J.) null
Sawako Yamashiro et al.
The Journal of biological chemistry, 289(17), 11616-11629 (2014-03-20)
Tropomodulins (Tmods) are F-actin pointed end capping proteins that interact with tropomyosins (TMs) and cap TM-coated filaments with higher affinity than TM-free filaments. Here, we tested whether differences in recognition of TM or actin isoforms by Tmod1 and Tmod3 contribute
Maria Sckolnick et al.
Molecular biology of the cell, 27(19), 2889-2897 (2016-08-19)
Tropomyosin (Tpm) isoforms decorate actin with distinct spatial and temporal localization patterns in cells and thus may function to sort actomyosin processes by modifying the actin track affinity for specific myosin isoforms. We examined the effect of three Tpm isoforms
Ju Lan Chun et al.
Stem cells translational medicine, 2(1), 68-80 (2013-01-04)
Duchenne muscular dystrophy (DMD) is the most common form of muscular dystrophy. DMD patients lack dystrophin protein and develop skeletal muscle pathology and dilated cardiomyopathy (DCM). Approximately 20% succumb to cardiac involvement. We hypothesized that mesoangioblast stem cells (aorta-derived mesoangioblasts

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