MABS829

Anti-phospho-EGFR (Tyr1173) Antibody, clone 3E7.2

clone 3E7.2, from mouse

• Sinonimo/i: Epidermal growth factor receptor, EC: 2.7.10.1, Proto-oncogene c-ErbB1, Receptor tyrosine protein kinase erbB-1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 3E7.2, monoclonal
• Reattività contro le specie: human
• tecniche: immunocytochemistry: suitable; western blot: suitable
• Isotipo: IgG2bκ
• N° accesso NCBI: NP_005219
• N° accesso UniProt: P00533
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: phosphorylation (pTyr1173)
• Informazioni sul gene: human ... EGFR(1956)

Descrizione

Descrizione generale

Epidermal growth factor receptor (UniProt: P00533; also known as EC: 2.7.10.1; Proto-oncogene c-ErbB1, Receptor tyrosine protein kinase erbB-1) is encoded by the EGFR (also known as ERBB, ERBB1, HER1) gene (Gene ID: 1956) in human. EGFR is activated by binding of its specific ligands and transforms from a monomeric to active homodimeric form. In addition to forming homodimers, EGFR may pair with another member of the ErbB receptor family, such as ErbB2/Her2/neu, to create an activated heterodimer. EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity. As a result, autophosphorylation of five tyrosine residues (Y992, Y1045, Y1068, Y1148 and Y1173) occurs in the C-terminal domain. This autophosphorylation elicits downstream activation and signaling by several other proteins that associate with the phosphorylated tyrosines through their own phosphotyrosine-binding domains. EGFR activation results in at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC and STATs modules. These downstream events modulate several cell functions, including migration, adhesion, and proliferation. Endocytosis and inhibition of the activated EGFR by phosphatases constitute key regulatory mechanisms.

~200 kDa observed; 134.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Immunogeno

KLH-conjugated linear peptide corresponding to 13 amino acids surrrounding Tyrosine 1173 of human epidermal growth factor receptor.

Applicazioni

Detect phospho EGFR using this mouse monoclonal Anti-phospho-EGFR (Tyr1173), clone 3E7.2, Cat. No. MABS829, used in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected phospho-EGFR (Tyr1173) in A431 cells treated with EGF.

Stato fisico

Format: Purified

Risultati analitici

Evaluated by Western Blotting in A431 cells treated with EGF.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected phospho-EGFR (Tyr1173) in 10 µg lysate from A431 cells treated with EGF.

Altre note

Concentration: Please refer to lot specific datasheet.

Replaces: 05-483

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1