OP43

Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1)

liquid, clone DO-1, Calbiochem^®

• Sinonimo/i: Anti-p53 Antibody, Mouse Anti-p53, p53 Detection Antibody
• Codice UNSPSC: 12352203
• NACRES: NA.43

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: DO-1, monoclonal
• Stato: liquid
• contiene: ≤0.1% sodium azide as preservative
• Reattività contro le specie: feline, human
• Non deve reagire con: mouse, rat
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: do not freeze
• dilution: (Frozen sections (1 µg/mL) ; Immunoblotting (0.1-1 µg/mL); Immunocytochemistry (1-2.5 µg/mL); Immunoprecipitation (1 µg/mL) ; Paraffin sections (1 µg/mL, pepsin, heat or pressure cooker pre-treatment required))
• Isotipo: IgG2a
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... TP53(7157)

Descrizione

Descrizione generale

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with X63Ag8.653 mouse myeloma cells (see application references). Recognizes the ~53 kDa wild-type and mutant forms of p53.

Recognizes the ~53 kDa wild-type and mutant p53 protein in A431 cells and breast carcinoma tissue.

This Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) is validated for use in Frozen sections, Immunoblotting, ICC, IP, Paraffin sections for the detection of p53 (Ab-6) (Pantropic).

Immunogeno

Epitope: Within amino acids 21-25 of human p53

Human

wild type, recombinant, human p53

Applicazioni

Frozen sections (1 g/ml; see application references)
Immunoblotting (0.1-1 g/ml; see application references)
Immunocytochemistry (1-2.5 g/ml; see application references)
Immunoprecipitation (1 g/ml or use Cat. No. OP43A; see application references)
Paraffin sections (1 g/ml, pepsin, heat or pressure cooker pre-treatment required; see application references)

Confezionamento

Please refer to vial label for lot-specific concentration.

Stato fisico

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

Risultati analitici

Negative Control
SK-OV-3 cells or normal skin tissue

Positive Control
A431 cells or breast carcinoma tissue

Altre note

El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Legros, Y., et al. 1994. Oncogene9, 2071.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kastan, M.B., et al. 1992. Cell71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Lane, D.P. 1992. Nature358, 15.
Vojtesek, B., et al. 1992. J. Immunol. Meth.151, 237.
Kastan, M.B., et al. 1991 Cancer Res.51, 6304.

Recognizes both mutant and wild-type p53 under denaturing and non-denaturing conditions. This antibody reacts weakly with rodent p53; we do not recommend it for rodent samples. For gel shift assay, use Cat. No. OP43L resuspended in 100 μl of buffer. Wild-type p53 has a short half life and is present in low amounts in cells. For immunoprecipitation increasing the amount of sample and labeling with ³⁵S-Met for less than or equal to 1 h will aid in visualizing wild-type p53. Antibody should be titrated for optimal results in individual systems.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Toxicity: Standard Handling (A)

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable