QIA127

Rapid Cell Proliferation Kit

• Sinonimo/i: Proliferation assay kit
• Codice UNSPSC: 12352207
• NACRES: NA.84

Proprietà

• impiego: sufficient for 500 tests
• Livello qualitativo: 100
• Confezionamento: pkg of 1 96-well plate(s)
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; avoid repeated freeze/thaw cycles; protect from light
• input: sample type cultured cells (1,000-50,000 cells)
• Metodo di rivelazione: colorimetric
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Assay measures the increased activity of cellular mitochondrial dehydrogenases that can cleave the tetrazolium dye WST-1 to formazan. The formazan formation is then quantified by measuring the change in absorbance at 450 nm in a microplate reader. The activity of mitochondrial dehydrogenases is proportional to cell number. No washing, harvesting, or solubilization steps are required.

Azioni biochim/fisiol

The Calbiochem Rapid Cell Proliferation Kit is designed to determine the number of viable cells in proliferation, or cytotoxicity studies, based on the cleavage of the tetrazolium salt WST-1 by mitochondrial dehydrogenases in viable cells. The assay can be used to measure cell proliferation in response to growth factors or cytokines, or to assess cytotoxic compounds.

Caratteristiche e vantaggi

Assay Time: 0.5 - 4 h

Sensitivity: 1000 cells (adherent); 2500 cells (nonadherent)

Nota sulla preparazione

• WST-1 Labeling Mixture: Thaw the EMS solution at room temperature or in a 37°C water bath. Add the entire contents of this solution to the bottle containing the WST-1 reagent. The mixed solution can be stored at 4°C for several weeks. For long-term storage (several months) aliquot and store at -20°C. Protect from light.

Altre note

Ishiyama, M., et al. 1995. In Vitro Toxicology8, 187.
Liu, S.O., et al 1995. Nat. Med.1, 267.
Ishiyama, M., et al. 1993. Chem. Pharm. Bull.41, 1118.

WST-1, 1-Methoxy PMS, and a user protocol.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids