Passa al contenuto
Merck

4352157

Trypsin, TPCK-Treated

Sinonimo/i:

6- (1-tosylamido-2-phenyl) ethyl chloromethyl ketone treated Trypsin, Modified trypsin (TPCK-Treated), Trypsin, TPCK treated

Slide 1 of 1
Autenticati per visualizzare i prezzi riservati alla tua organizzazione & contrattuali

Scegli un formato

1 KIT

208,00 €

208,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Richiedi un ordine in blocco

Informazioni su questo articolo

Codice UNSPSC:
12352204
NACRES:
NA.56

Documenti fondamentali

Visualizza tutta la documentazione

Vai a

Servizio Tecnico
Hai bisogno di aiuto? Il nostro team di scienziati qualificati è a tua disposizione.
Permettici di aiutarti

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Descrizione generale

Trypsin is a serine protease that specifically hydrolyzes peptide bonds at the carboxyl side of Lysine and Arginine residues. This modified trypsin has been treated with N-tosyl-L-phenylalanine chloromethyl ketone (TPCK) to inactivate extraneous chymotryptic activity. This trypsin product also contains calcium chloride, which may improve consistency in digestion. Each package contains 10 vials, with 25 μg in each vial. 

Applicazioni

Trypsin, TPCK-Treated has been used for the digestion of protein samples to generate peptides for LC-MS (liquid chromatography–mass spectrometry)/MS analysis.[1][2][3]
Trypsin, TPCK-Treated has been used in enzymatic treatment to cleave the recombinant native B-cell lymphoma-extra large (Bcl-xL)-ΔTM monomer to generate fibrils (the third type of Bcl-xL fibril, called “BclxLcf37”).[4] It has also been used in influenza infection experiments as a supplement in infection/DMEM serum-free medium to infect A549 cells with influenza A H1N1 virus at a multiplicity of infection (MOI) to perform multi-round infection.[5]

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H315,H319,H334

Classi di pericolo

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2

Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documenti section.

Se ti serve aiuto, non esitare a contattarci Servizio Clienti

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Pavel Bouchal et al.
Cell reports, 28(3), 832-843 (2019-07-18)
Accurate classification of breast tumors is vital for patient management decisions and enables more precise cancer treatment. Here, we present a quantitative proteotyping approach based on sequential windowed acquisition of all theoretical fragment ion spectra (SWATH) mass spectrometry and establish
Quantitative milk proteomics--host responses to lipopolysaccharide-mediated inflammation of bovine mammary gland.
Danielsen M, et al.
Proteomics, 10, 2240-2249 (2010)
Alexis Gonneaud et al.
International journal of molecular sciences, 21(20) (2020-10-21)
Bcl-xL, a member of the Bcl-2 family, is a pro-survival protein involved in apoptosis regulation. We have previously reported the ability of Bcl-xL to form various types of fibers, from native to amyloid conformations. Here, we have mimicked the effect
Irene Pila-Castellanos et al.
PLoS pathogens, 17(2), e1009340-e1009340 (2021-02-18)
Influenza virus infections are major public health threats due to their high rates of morbidity and mortality. Upon influenza virus entry, host cells experience modifications of endomembranes, including those used for virus trafficking and replication. Here we report that influenza
Douglas E Vetter et al.
Methods in molecular biology (Clifton, N.J.), 493, 345-366 (2008-10-08)
Modern biologists have at their disposal a large array of techniques used to assess the existence and relative or absolute quantity of any molecule of interest in a sample. However, implementing most of these procedures can be a daunting task
Visualizza tutti i documenti correlati

Domande

1–2 di 2 domande  

  1. What is the most suitable buffer to reconstitute it considering a protocol to activate influenza virus prior to mammalian culture infection?

    1 risposta

    1. This product has been used in influenza infection experiments as a supplement in the infection medium. The medium used is DMEM serum-free medium supplemented with 50 IU/ml penicillin, 50 mg/ml streptomycin, and 0.2 mg/ml TPCK-trypsin. Relevant references include:
      "Mitochondrial morphodynamics alteration induced by influenza virus infection as a new antiviral strategy," PLoS Pathogens (2021-02-18), PMID: 33596274, Irene Pila-Castellanos, Diana Molino, Joe McKellar, et al.
      "Viruses traverse the human proteome through peptide interfaces that can be biomimetically leveraged for drug discovery," L Meyniel-Schicklin, J Amaudrut… - Proceedings of the National Academy of Sciences, 2024.

      Utile?

  2. Hi, We wonder how is the best buffer or solution to resuspend this product?. Cat 352157 Sigma-Aldrich. Trypsin, TPCK-Treated

    1 risposta

    1. For the purpose of in-gel tryptic digestion, it is recommended to reconstitute a vial of trypsin with 1 mL of 100 mM ammonium bicarbonate (NH4HCO3) pH 8.5.

      Utile?

Recensioni

Nessuna valutazione

Filtri attivi

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica