It is not possible to have cells that are PI positive without the cells also being positive for Annexin V binding. Cells are PI positive because the membrane has been compromised. If this is the case, Annexin V can also enter the cell and bind to the PS on the internal cell membrane. The gating on the histogram for the FITC channel should be changed so that all cells that are PI positive are also Annexin V positive.
APOAF
Kit per il rilevamento dell′apoptosi con annessina V-FITC
Sinonimo/i:
Annexin V-FITC, Apoptosis probe FITC
Autenticati per visualizzare i prezzi organizzativi e contrattuali.
Scegli un formato
20 TEST
342,00 €
342,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Informazioni su questo articolo
NACRES:
NA.84
UNSPSC Code:
12352207
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Permettici di aiutartiusage
(20 tests)
packaging
pkg of 1 kit
technique(s)
flow cytometry: suitable
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Categorie correlate
1 of 4
Questo articolo | APOAC | S7111 | QIA39 |
|---|---|---|---|
| technique(s) flow cytometry: suitable | technique(s) flow cytometry: suitable | technique(s) flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | technique(s) flow cytometry: suitable |
| usage (20 tests) | usage sufficient for 200 tests | usage - | usage sufficient for 50 tests |
| packaging pkg of 1 kit | packaging pkg of 1 kit | packaging - | packaging - |
| application(s) cell analysis | application(s) cell analysis | application(s) - | application(s) - |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
| shipped in wet ice | shipped in wet ice | shipped in dry ice | shipped in wet ice |
Application
Il kit per il rilevamento dell′apoptosi con annessina V-FITC è stato usato:
- nella colorazione di cellule di cancro della prostata LNCaP per misurare l′attività di estratti di G. lucidum durante il trattamento del cancro della prostata.[1]
- per la marcatura di cellule tumorali per studiare l′attività inibitoria di DBP-maf (proteina legante la vitamina D-fattore di attivazione dei macrofagi) su cellule tumorali prostatiche.[2]
- per la misurazione indiretta dell′attività della flippasi.[3]
Features and Benefits
Rileva l′apoptosi più precocemente nel corso della procedura rispetto ai saggi basati sul DNA, come TUNEL.
- Rapida marcatura delle cellule. La colorazione cellulare richiede solo 10 minuti.
- Non è necessario fissare o trattare le cellule, per cui il tempo di rilevamento si riduce ed è possibile usare le cellule per un ulteriore studio.
- Nel kit è incluso il colorante secondario ioduro di propidio per differenziare le cellule apoptotiche da quelle vitali e necrotiche.
General description
Il kit dell′annessina V-FITC consente il rilevamento per fluorescenza dell′annessina V legata alle cellule apoptotiche e la determinazione quantitativa tramite citometria a flusso. Il kit dell′annessina V-FITC usa l′annessina V coniugata con isotiocianato di fluoresceina (FITC) per marcare i siti della fosfatidilserina sulla superficie della membrana. Il kit include lo ioduro di propidio (PI) per marcare il DNA cellulare nelle cellule necrotiche in cui la membrana cellulare è stata totalmente compromessa. Questa combinazione consente di differenziare le cellule in fase apoptotica iniziale (positive all′annessina V, negative al PI), le cellule necrotiche (positive all′annessina V, positive al PI) e le cellule vitali (negative all′annessina V, negative al PI).
Other Notes
Lasciare raggiungere la temperatura ambiente a tutti i componenti prima dell'uso.
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10 - Combustible liquids
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Contenuto correlato
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When using Product APOAF, Annexin V-FITC Apoptosis Detection Kit, I have cells that are Annexin V FITC negative and PI positive. What are these cells?
1 risposta-
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Can I use Product APOAF, Annexin V-FITC Apoptosis Detection Kit, to differentiate cells that are dead due to necrosis or apoptosis?
1 risposta-
When using a kinetic study (various time points), you can show the progression of the cells from viable (annexin V FITC negative, PI negative), to annexin FITC positive, PI negative (membrane flip) to annexin V FITC positive, PI positive (dead). If there are cells that are double positive when starting, it is not possible to guarantee that the cell death occurred due to apoptosis with this assay.
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What is the Department of Transportation shipping information for this product?
1 risposta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Utile?
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Can Product APOAF, Annexin V-FITC Apoptosis Detection Kit be used on fixed cells?
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No. Product APOAF, Annexin V-FITC Apoptosis Detection Kit must be performed on live cells in order to measure Apoptosis. The assay is based on the externization of phosphatidylserine from the inner cell membrane to the outer cell membrane. If the membrane is preturbed due to fixation, non-speciifc staining of the inner cell membrane might occur
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When using the Annexin V-FITC Apoptosis Detection Kit, Product APOAF, can I use any buffer for resuspending my cells?
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The binding buffer included in the kit needs to be used for resuspending cells. The buffer contains calcium chloride at a final (1X) concentration of 2.5 mM which is necessary for the binding of annexin V to phosphatidylserine.
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What wavelength do I use to detect Annexin V-FITC and Propidium Iodide when using Annexin V-FITC Apoptosis Detection Kit, Product APOAF?
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Annexin V FITC will have a maximum emission of 528 nm. This can be measured in the standard FITC Channel on a flow cytometer (FL1). Propidium Iodide has a maximum emission of 620 nm. This is measured on the short red channel on a flow cytometer (FL2 or FL3).
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