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Merck

GE17-0780-01

Protein A-Sepharose™ CL-4B

Cytiva 17-0780-01, pack of 1.5 g

Sinonimo/i:

Protein A Sepharose, Sepharose CL-4B

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1 PKG

667,00 €

667,00 €

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Informazioni su questo articolo

NACRES:
NA.56
UNSPSC Code:
41106500

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Nome del prodotto

Protein A-Sepharose™ CL-4B, Cytiva 17-0780-01, pack of 1.5 g

cleaning

2-10

pH

3—9

working range

3-9

ligand

native protein A

crosslinking

cross-linked

packaging

pack of 1.5 g

manufacturer/tradename

Cytiva 17-0780-01

storage condition

(20% Ehtanol)

parameter

150 cm/hr flow rate at 25 °C

matrix

4% cross-linked agarose

matrix active group

functional group

particle size

45-165 μm

average diameter

90 μm

capacity

~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

separation technique

affinity

storage temp.

2-8°C

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Sepharose™ CL-4B

Con-A Sepharose™ 4B Cytiva 17-0440-01, pack of 100 mL

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Con-A Sepharose™ 4B

matrix

4% cross-linked agarose

matrix

4% cross-linked agarose

matrix

4% agarose

matrix

4% agarose

particle size

45-165 μm

particle size

45-165 μm

particle size

45-165 μm

particle size

45-165 μm

capacity

~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

capacity

binding capacity(HSA (Mr 68 000) 15-20 mg/ml drained medium, ß-lactoglobulin (Mr 18 000) 3-5 mg/ml drained medium)

capacity

-

capacity

-

packaging

pack of 1.5 g

packaging

pack of 200 mL

packaging

pack of 1 L

packaging

pack of 100 mL

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

2-8°C

storage condition

(20% Ehtanol)

storage condition

-

storage condition

-

storage condition

(0.1 M acetate buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl, 20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

  • Well documented for immunoglobulin purification and fractionation
  • Strong binding to Fc region of IgG
  • High capacity - 20 mg human IgG/mL.
  • Suited for immunoprecipitation procedures

General description

Protein A Sepharose™ CL-4B is a classic, well documented medium for immunoglobin purification and fractionation, and is suitable for immunoprecipitation procedures.

Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose™ CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Legal Information

Sepharose is a trademark of Cytiva

Classe di stoccaggio

13 - Non Combustible Solids

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Certificati d'analisi (COA)

Lot/Batch Number

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